Location, Catalytic Activity, and Subunit Composition of NAD-reducing Hydrogenases of Some Alcaligenes Strains and Rhodococcus Opacus MR22

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1997 Feb 1

PMID 9133325

Citations 7

Authors

C Grzeszik

K Ross

K Schneider

M Reh

H G Schlegel

Affiliations

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Abstract

Six new strains of Alcaligenes enriched for and isolated as nickel-resistant bacteria resemble Alcaligenes eutrophus H16 and contain both an NAD-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. None of the soluble hydrogenases share with the Rhodococcus opacus MR11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an NADH:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. The soluble hydrogenase of the newly isolated strain MR22 of R. opacus equalled that of strain MR11. The absence of a membrane-bound hydrogenase in Alcaligenes denitrificans strain 4a-2 and in Alcaligenes ruhlandii was confirmed.

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