The Casein Kinase II Beta Subunit Binds to Mos and Inhibits Mos Activity

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1997 Apr 1

PMID 9121438

Citations 34

Authors

M Chen

D Li

E G KREBS

J A Cooper

Affiliations

Soon will be listed here.

Abstract

Mos is a germ cell-specific serine/threonine kinase and is required for Xenopus oocyte maturation. Active Mos stimulates a mitogen-activated protein kinase (MAPK) by directly phosphorylating and activating MAPK kinase (MKK). We report here that the Xenopus homolog of the beta subunit of casein kinase II (CKII beta) binds to and regulates Mos. The Mos-interacting region of CKII beta was mapped to the C terminus. Mos bound to CKII beta in somatic cells ectopically expressing Mos and CKII beta as well as in unfertilized Xenopus eggs. CKII beta inhibited Mos-mediated MAPK activation in rabbit reticulocyte lysates and repressed MKK activation by v-Mos in a coupled kinase assay. In addition, microinjection of CKII beta mRNA into Xenopus oocytes inhibited progesterone-induced meiotic maturation and MAPK activation, presumably by binding of CKII beta to Mos and thereby inhibiting MAPK activation. Moreover, this inhibitory phenotype could be rescued by another protein that binds to CKII beta, CKII alpha. The ability of ectopic CKII beta to inhibit meiotic maturation and the detection of a complex between endogenous Mos and CKII beta suggest that CKII beta may act as an inhibitor of Mos during oocyte maturation, perhaps setting a threshold beyond which Mos protein must accumulate before it can activate the MAPK pathway.

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References

Luscher B, Litchfield D . Biosynthesis of casein kinase II in lymphoid cell lines. Eur J Biochem. 1994; 220(2):521-6. DOI: 10.1111/j.1432-1033.1994.tb18651.x. View

Zheng C, Guan K . Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 1994; 13(5):1123-31. PMC: 394921. DOI: 10.1002/j.1460-2075.1994.tb06361.x. View

Alessi D, Saito Y, Campbell D, Cohen P, Sithanandam G, Rapp U . Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J. 1994; 13(7):1610-9. PMC: 394991. DOI: 10.1002/j.1460-2075.1994.tb06424.x. View

Mayer B, Baltimore D . Mutagenic analysis of the roles of SH2 and SH3 domains in regulation of the Abl tyrosine kinase. Mol Cell Biol. 1994; 14(5):2883-94. PMC: 358656. DOI: 10.1128/mcb.14.5.2883-2894.1994. View

Stigare J, Buddelmeijer N, PIGON A, Egyhazi E . A majority of casein kinase II alpha subunit is tightly bound to intranuclear components but not to the beta subunit. Mol Cell Biochem. 1993; 129(1):77-85. DOI: 10.1007/BF00926578. View

Kosako H, Gotoh Y, Nishida E . Requirement for the MAP kinase kinase/MAP kinase cascade in Xenopus oocyte maturation. EMBO J. 1994; 13(9):2131-8. PMC: 395066. DOI: 10.1002/j.1460-2075.1994.tb06489.x. View

Feilotter H, Hannon G, Ruddell C, Beach D . Construction of an improved host strain for two hybrid screening. Nucleic Acids Res. 1994; 22(8):1502-3. PMC: 308013. DOI: 10.1093/nar/22.8.1502. View

Marshall C . MAP kinase kinase kinase, MAP kinase kinase and MAP kinase. Curr Opin Genet Dev. 1994; 4(1):82-9. DOI: 10.1016/0959-437x(94)90095-7. View

Gotoh Y, Matsuda S, Takenaka K, Hattori S, Iwamatsu A, Ishikawa M . Characterization of recombinant Xenopus MAP kinase kinases mutated at potential phosphorylation sites. Oncogene. 1994; 9(7):1891-8. View

10.

Colledge W, Carlton M, Udy G, Evans M . Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs. Nature. 1994; 370(6484):65-8. DOI: 10.1038/370065a0. View

11.

Hashimoto N, Watanabe N, Furuta Y, Tamemoto H, Sagata N, Yokoyama M . Parthenogenetic activation of oocytes in c-mos-deficient mice. Nature. 1994; 370(6484):68-71. DOI: 10.1038/370068a0. View

12.

Selfors L, Stern M . MAP kinase function in C. elegans. Bioessays. 1994; 16(5):301-4. DOI: 10.1002/bies.950160502. View

13.

Mansour S, Matten W, Hermann A, Candia J, Rong S, Fukasawa K . Transformation of mammalian cells by constitutively active MAP kinase kinase. Science. 1994; 265(5174):966-70. DOI: 10.1126/science.8052857. View

14.

Wang X, Yew N, Peloquin J, Woude G, Borisy G . Mos oncogene product associates with kinetochores in mammalian somatic cells and disrupts mitotic progression. Proc Natl Acad Sci U S A. 1994; 91(18):8329-33. PMC: 44599. DOI: 10.1073/pnas.91.18.8329. View

15.

Turner D, Weintraub H . Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate. Genes Dev. 1994; 8(12):1434-47. DOI: 10.1101/gad.8.12.1434. View

16.

Sheets M, Fox C, Hunt T, Vande Woude G, Wickens M . The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation. Genes Dev. 1994; 8(8):926-38. DOI: 10.1101/gad.8.8.926. View

17.

Bilger A, Fox C, Wahle E, Wickens M . Nuclear polyadenylation factors recognize cytoplasmic polyadenylation elements. Genes Dev. 1994; 8(9):1106-16. DOI: 10.1101/gad.8.9.1106. View

18.

Fields S, Sternglanz R . The two-hybrid system: an assay for protein-protein interactions. Trends Genet. 1994; 10(8):286-92. DOI: 10.1016/0168-9525(90)90012-u. View

19.

Resing K, Mansour S, Hermann A, Johnson R, Candia J, Fukasawa K . Determination of v-Mos-catalyzed phosphorylation sites and autophosphorylation sites on MAP kinase kinase by ESI/MS. Biochemistry. 1995; 34(8):2610-20. DOI: 10.1021/bi00008a027. View

20.

Allende J, Allende C . Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation. FASEB J. 1995; 9(5):313-23. DOI: 10.1096/fasebj.9.5.7896000. View