The K43 Gene, Required for Chorion Gene Amplification and Diploid Cell Chromosome Replication, Encodes the Drosophila Homolog of Yeast Origin Recognition Complex Subunit 2

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1997 Apr 15

PMID 9108074

Citations 52

Authors

G Landis

R Kelley

A C Spradling

J Tower

Affiliations

Soon will be listed here.

Abstract

Lethal alleles of the Drosophila k43 gene result in small or missing imaginal discs, greatly reduced mitotic index, and fragmented and abnormally condensed chromosomes. A female-sterile allele of k43 specifically reduces chorion gene amplification in ovarian follicle cells. k43 was cloned by chromosomal walking, and the identification of the k43 gene was confirmed by phenotypic rescue and sequence analysis of mutant alleles. The sequence analyses reveal that the k43 gene encodes the Drosophila homolog of the yeast origin recognition complex subunit 2 (Orc2p), a protein required for replication origin function and transcriptional silencing in yeast. These results suggest an evolutionarily conserved role for Orc2p in eukaryotic chromosomal DNA replication.

Citing Articles

Tissue-Specific DNA Replication Defects in Caused by a Meier-Gorlin Syndrome Mutation in Orc4.

McDaniel S, Hollatz A, Branstad A, Gaskill M, Fox C, Harrison M Genetics. 2019; 214(2):355-367.

PMID: 31818869 PMC: 7017028. DOI: 10.1534/genetics.119.302938.

Rapid DNA Synthesis During Early Embryogenesis Is Sensitive to Maternal Humpty Dumpty Protein Function.

Lesly S, Bandura J, Calvi B Genetics. 2017; 207(3):935-947.

PMID: 28942426 PMC: 5676233. DOI: 10.1534/genetics.117.300318.

DNA Replication Control During Development: Insights into the Onset of S Phase, Replication Initiation, and Fork Progression.

Hua B, Orr-Weaver T Genetics. 2017; 207(1):29-47.

PMID: 28874453 PMC: 5586379. DOI: 10.1534/genetics.115.186627.

Polyteny: still a giant player in chromosome research.

Stormo B, Fox D Chromosome Res. 2017; 25(3-4):201-214.

PMID: 28779272 PMC: 5768140. DOI: 10.1007/s10577-017-9562-z.

Mechanisms and regulation of DNA replication initiation in eukaryotes.

Parker M, Botchan M, Berger J Crit Rev Biochem Mol Biol. 2017; 52(2):107-144.

PMID: 28094588 PMC: 5545932. DOI: 10.1080/10409238.2016.1274717.

References

de Cicco D, Spradling A . Localization of a cis-acting element responsible for the developmentally regulated amplification of Drosophila chorion genes. Cell. 1984; 38(1):45-54. DOI: 10.1016/0092-8674(84)90525-7. View

Kayne P, Kim U, Han M, Mullen J, Yoshizaki F, Grunstein M . Extremely conserved histone H4 N terminus is dispensable for growth but essential for repressing the silent mating loci in yeast. Cell. 1988; 55(1):27-39. DOI: 10.1016/0092-8674(88)90006-2. View

CARPENTER P, Mueller P, Dunphy W . Role for a Xenopus Orc2-related protein in controlling DNA replication. Nature. 1996; 379(6563):357-60. DOI: 10.1038/379357a0. View

Swimmer C, Delidakis C, Kafatos F . Amplification-control element ACE-3 is important but not essential for autosomal chorion gene amplification. Proc Natl Acad Sci U S A. 1989; 86(22):8823-7. PMC: 298382. DOI: 10.1073/pnas.86.22.8823. View

. Drosophila chorion genes: cracking the eggshell's secrets. Bioessays. 1991; 13(3):97-105. DOI: 10.1002/bies.950130302. View

Heck M, Spradling A . Multiple replication origins are used during Drosophila chorion gene amplification. J Cell Biol. 1990; 110(4):903-14. PMC: 2116105. DOI: 10.1083/jcb.110.4.903. View

Gatti M, Baker B . Genes controlling essential cell-cycle functions in Drosophila melanogaster. Genes Dev. 1989; 3(4):438-53. DOI: 10.1101/gad.3.4.438. View

Romanowski P, Madine M, Rowles A, J Blow J, Laskey R . The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin. Curr Biol. 1996; 6(11):1416-25. DOI: 10.1016/s0960-9822(96)00746-4. View

Johnston C, Spradling A . The role of ACE3 in Drosophila chorion gene amplification. EMBO J. 1989; 8(13):4153-62. PMC: 401604. DOI: 10.1002/j.1460-2075.1989.tb08600.x. View

10.

Foss M, McNally F, Laurenson P, Rine J . Origin recognition complex (ORC) in transcriptional silencing and DNA replication in S. cerevisiae. Science. 1993; 262(5141):1838-44. DOI: 10.1126/science.8266071. View

11.

Cooley L, Kelley R, Spradling A . Insertional mutagenesis of the Drosophila genome with single P elements. Science. 1988; 239(4844):1121-8. DOI: 10.1126/science.2830671. View

12.

Takahara K, Bong M, Brevard R, Eddy R, Haley L, Sait S . Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L. Genomics. 1996; 31(1):119-22. DOI: 10.1006/geno.1996.0018. View

13.

Shearn A, Garen A . Genetic control of imaginal disc development in Drosophila. Proc Natl Acad Sci U S A. 1974; 71(4):1393-7. PMC: 388235. DOI: 10.1073/pnas.71.4.1393. View

14.

Diffley J, Cocker J . Protein-DNA interactions at a yeast replication origin. Nature. 1992; 357(6374):169-72. DOI: 10.1038/357169a0. View

15.

Fox C, Loo S, Dillin A, Rine J . The origin recognition complex has essential functions in transcriptional silencing and chromosomal replication. Genes Dev. 1995; 9(8):911-24. DOI: 10.1101/gad.9.8.911. View

16.

Szabad J, Bryant P . The mode of action of "discless" mutations in Drosophila melanogaster. Dev Biol. 1982; 93(1):240-56. DOI: 10.1016/0012-1606(82)90256-1. View

17.

Delidakis C, Kafatos F . Amplification enhancers and replication origins in the autosomal chorion gene cluster of Drosophila. EMBO J. 1989; 8(3):891-901. PMC: 400889. DOI: 10.1002/j.1460-2075.1989.tb03450.x. View

18.

Bell S, Stillman B . ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature. 1992; 357(6374):128-34. DOI: 10.1038/357128a0. View

19.

Underwood E, Briot A, Doll K, Ludwiczak R, Otteson D, Tower J . Genetics of 51D-52A, a region containing several maternal-effect genes and two maternal-specific transcripts in Drosophila. Genetics. 1990; 126(3):639-50. PMC: 1204219. DOI: 10.1093/genetics/126.3.639. View

20.

Bell S, Kobayashi R, Stillman B . Yeast origin recognition complex functions in transcription silencing and DNA replication. Science. 1993; 262(5141):1844-9. DOI: 10.1126/science.8266072. View