Population Structure and Physiological Changes Within a Hot Spring Microbial Mat Community Following Disturbance

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1997 Apr 1

PMID 9097433

Citations 23

Authors

M J Ferris

S C Nold

N P Revsbech

D M Ward

Affiliations

Soon will be listed here.

Abstract

The influence of disturbance on a hot spring cyanobacterial mat community was investigated by physically removing the top 3.0 mm, which included the entire cyanobacterial layer. Changes in 16S rRNA-defined populations were monitored by denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rRNA gene segments. Some previously absent cyanobacterial populations colonized the disturbed areas, while some populations which were present before the disturbance remained absent for up to 40 days. Changes in physiological activity were measured by oxygen microelectrode analyses and by 14CO2 incorporation into cyanobacterial molecular components. These investigations indicated substantial differences between the disturbed and undisturbed mats, including an unexplained light-induced oxygen consumption in the freshly exposed mat, increased carbon partitioning by phototrophs into growth-related macromolecules, bimodal vertical photosynthesis profiles, and delayed recovery of respiration relative to photosynthesis.

Citing Articles

Polyphasic characterization of a novel hot-spring cyanobacterium gen et sp. nov. and genomic insights into its carbon concentration mechanism.

Jiang Y, Tang J, Liu X, Daroch M Front Microbiol. 2023; 14:1176500.

PMID: 37564287 PMC: 10410155. DOI: 10.3389/fmicb.2023.1176500.

Differential Phototactic Behavior of Closely Related Cyanobacterial Isolates from Yellowstone Hot Spring Biofilms.

Bunbury F, Rivas C, Calatrava V, Shelton A, Grossman A, Bhaya D Appl Environ Microbiol. 2022; 88(10):e0019622.

PMID: 35499327 PMC: 9128501. DOI: 10.1128/aem.00196-22.

Relationship between Microorganisms Inhabiting Alkaline Siliceous Hot Spring Mat Communities and Overflowing Water.

Becraft E, Jackson B, Nowack S, Klapper I, Ward D Appl Environ Microbiol. 2020; 86(23).

PMID: 32978131 PMC: 7657625. DOI: 10.1128/AEM.00194-20.

The Dark Side of the Mushroom Spring Microbial Mat: Life in the Shadow of Chlorophototrophs. I. Microbial Diversity Based on 16S rRNA Gene Amplicons and Metagenomic Sequencing.

Thiel V, Wood J, Olsen M, Tank M, Klatt C, Ward D Front Microbiol. 2016; 7:919.

PMID: 27379049 PMC: 4911352. DOI: 10.3389/fmicb.2016.00919.

The molecular dimension of microbial species: 2. Synechococcus strains representative of putative ecotypes inhabiting different depths in the Mushroom Spring microbial mat exhibit different adaptive and acclimative responses to light.

Nowack S, Olsen M, Schaible G, Becraft E, Shen G, Klapper I Front Microbiol. 2015; 6:626.

PMID: 26175719 PMC: 4484337. DOI: 10.3389/fmicb.2015.00626.

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