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Characterization of Polymorphisms in the Toxin A and B Genes of Clostridium Difficile

Overview
Journal FEMS Microbiol Lett
Publisher Oxford University Press
Specialty Microbiology
Date 1997 Mar 15
PMID 9084148
Citations 31
Authors
M Rupnik
V Braun
F Soehn
M Janc
M Hofstetter
R Laufenberg-Feldmann
C von Eichel-Streiber
Affiliations
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Abstract

We have used six independent polymerase chain reactions (A1-A3 and B1-B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5' third of tcdB (B1 fragment) and/or the 3' third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.

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