Altered Expression of Alpha 4 Beta 7, a Gut Homing Integrin, by Circulating and Mucosal T Cells in Colonic Mucosal Inflammation

Overview

Journal Gut

Specialty Gastroenterology

Date 1997 Feb 1

PMID 9071939

Citations 34

Authors

J Meenan

J Spaans

T A Grool

S T Pals

G N Tytgat

S J van Deventer

Affiliations

Soon will be listed here.

Abstract

Background And Aims: Expression of alpha 4 beta 7 on memory T lymphocytes identifies a cell population that preferentially migrates to the gut. Detection of alpha 4 beta 7 on circulating lymphocytes may permit the identification of specific subsets trafficking between the circulation and the gut in inflammatory bowel diseases.

Patients: Samples and clinical details were taken from patients with Crohn's disease (CD), ulcerative colitis (UC), diverticulitis/ infectious colitis, and healthy controls.

Methods: Peripheral blood and lamina propria mononuclear cells were isolated. Cells were labelled with CD3, CD4, CD25, CD45RO or alpha 4 beta 7.

Results: Median levels of circulating total memory T cells (CD4+CD45RO+) were increased in CD (p < 0.01) and UC (p < 0.05). However, the proportion of systemic gut homing T cells (CD4+CD45RO+ alpha 4 beta 7+) was decreased in CD (p < 0.05), UC (p < 0.002), and inflammatory controls (p < 0.05). Levels of activated gut homing T cells (CD4+CD25+ alpha 4 beta 7+) were increased in CD (p < 0.01) and UC (p < 0.05). For both CD4+CD45RO+ and CD4+CD25+ cells, the proportion of lymphocytes coexpressing alpha 4 beta 7 was decreased compared with controls. In small and large intestine lamina propria, expression of alpha 4 beta 7+ on CD3+ cells was extensive, although it was decreased in CD (p < 0.03), UC (p < 0.05), and inflammatory controls (p < 0.05).

Conclusions: Circulating and mucosal gut homing lymphocyte populations are changed in patients with colonic inflammation. This may arise due to a dilution effect from recruited naive T cells, or from integrin down regulation. Changes in general CD4+ lymphocyte populations mask more subtle variations in those cells with gut homing potential.

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References

Sadlack B, Merz H, Schorle H, Schimpl A, Feller A, Horak I . Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene. Cell. 1993; 75(2):253-61. DOI: 10.1016/0092-8674(93)80067-o. View

Conlon K, Osborne J, Morimoto C, Ortaldo J, Young H . Comparison of lymphokine secretion and mRNA expression in the CD45RA+ and CD45RO+ subsets of human peripheral blood CD4+ and CD8+ lymphocytes. Eur J Immunol. 1995; 25(3):644-8. DOI: 10.1002/eji.1830250303. View

Springer T . Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-14. DOI: 10.1016/0092-8674(94)90337-9. View

MacDonald T, Murch S . Aetiology and pathogenesis of chronic inflammatory bowel disease. Baillieres Clin Gastroenterol. 1994; 8(1):1-34. DOI: 10.1016/s0950-3528(06)80017-5. View

Erle D, Briskin M, Butcher E, Garcia-Pardo A, Lazarovits A, Tidswell M . Expression and function of the MAdCAM-1 receptor, integrin alpha 4 beta 7, on human leukocytes. J Immunol. 1994; 153(2):517-28. View

Yacyshyn B, Lazarovits A, Tsai V, Matejko K . Crohn's disease, ulcerative colitis, and normal intestinal lymphocytes express integrins in dissimilar patterns. Gastroenterology. 1994; 107(5):1364-71. DOI: 10.1016/0016-5085(94)90538-x. View

Pals S, Drillenburg P, Dragosics B, Lazarovits A, Radaszkiewicz T . Expression of the mucosal homing receptor alpha 4 beta 7 in malignant lymphomatous polyposis of the intestine. Gastroenterology. 1994; 107(5):1519-23. DOI: 10.1016/0016-5085(94)90558-4. View

Sparshott S, Bell E . Membrane CD45R isoform exchange on CD4 T cells is rapid, frequent and dynamic in vivo. Eur J Immunol. 1994; 24(11):2573-8. DOI: 10.1002/eji.1830241102. View

Rudolphi A, Boll G, Poulsen S, Claesson M, Reimann J . Gut-homing CD4+ T cell receptor alpha beta+ T cells in the pathogenesis of murine inflammatory bowel disease. Eur J Immunol. 1994; 24(11):2803-12. DOI: 10.1002/eji.1830241134. View

10.

de Dombal F, Burton I, Clamp S, Goligher J . Short-term course and prognosis of Crohn's disease. Gut. 1974; 15(6):435-43. PMC: 1413010. DOI: 10.1136/gut.15.6.435. View

11.

James S . Remission of Crohn's disease after human immunodeficiency virus infection. Gastroenterology. 1988; 95(6):1667-9. DOI: 10.1016/s0016-5085(88)80094-5. View

12.

Fiocchi C . Immune events associated with inflammatory bowel disease. Scand J Gastroenterol Suppl. 1990; 172:4-12. DOI: 10.3109/00365529009091902. View

13.

Senju M, Hulstaert F, Lowder J, Jewell D . Flow cytometric analysis of peripheral blood lymphocytes in ulcerative colitis and Crohn's disease. Gut. 1991; 32(7):779-83. PMC: 1378995. DOI: 10.1136/gut.32.7.779. View

14.

Senju M, Wu K, Mahida Y, Jewell D . Two-color immunofluorescence and flow cytometric analysis of lamina propria lymphocyte subsets in ulcerative colitis and Crohn's disease. Dig Dis Sci. 1991; 36(10):1453-8. DOI: 10.1007/BF01296815. View

15.

Pirzer U, Schonhaar A, Fleischer B, Hermann E, Meyer zum Buschenfelde K . Reactivity of infiltrating T lymphocytes with microbial antigens in Crohn's disease. Lancet. 1991; 338(8777):1238-9. DOI: 10.1016/0140-6736(91)92104-a. View

16.

Nakamura M, Saito H, Kasanuki J, Tamura Y, Yoshida S . Cytokine production in patients with inflammatory bowel disease. Gut. 1992; 33(7):933-7. PMC: 1379407. DOI: 10.1136/gut.33.7.933. View

17.

Koizumi M, King N, LOBB R, Benjamin C, Podolsky D . Expression of vascular adhesion molecules in inflammatory bowel disease. Gastroenterology. 1992; 103(3):840-7. DOI: 10.1016/0016-5085(92)90015-q. View

18.

Schweighoffer T, Tanaka Y, Tidswell M, Erle D, Horgan K, Luce G . Selective expression of integrin alpha 4 beta 7 on a subset of human CD4+ memory T cells with Hallmarks of gut-trophism. J Immunol. 1993; 151(2):717-29. View

19.

Postigo A, Sanchez-Mateos P, Lazarovits A, Sanchez-Madrid F, de Landazuri M . Alpha 4 beta 7 integrin mediates B cell binding to fibronectin and vascular cell adhesion molecule-1. Expression and function of alpha 4 integrins on human B lymphocytes. J Immunol. 1993; 151(5):2471-83. View

20.

Kirman I, Nielsen O, Kjaersgaard E, Brynskov J . Interleukin-2 receptor alpha and beta chain expression by circulating alpha beta and gamma delta T cells in inflammatory bowel disease. Dig Dis Sci. 1995; 40(2):291-5. DOI: 10.1007/BF02065412. View