Thermal Cycle Dideoxy DNA Sequencing

Overview

Journal Mol Biotechnol

Publisher Springer

Specialties Biotechnology
Molecular Biology

Date 1996 Dec 1

PMID 9067976

Citations 1

Authors

B E Slatko

Affiliations

Soon will be listed here.

Abstract

Thermal cycle dideoxy DNA sequencing eliminates the requirements for independent primer annealing and double-stranded DNA denaturation steps. The method enables sequencing from nanogram amounts of DNA from double-stranded and single-stranded PCR products, and plasmid or phage DNA templates. Thermal cycle sequencing also enables direct sequencing from bacterial colonies or phage plaques. Protocols using the Vent exo- DNA polymerase, helpful suggestions, and a troubleshooting guide are also presented.

Citing Articles

Recent advances in molecular biological techniques and their relevance to pulmonary research.

Robinson B, Erle D, Jones D, Shapiro S, Metzger W, Albelda S Thorax. 2000; 55(4):329-39.

PMID: 10722774 PMC: 1745722. DOI: 10.1136/thorax.55.4.329.

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