Polarization of Adenosine Effects on Intracellular PH in A6 Renal Epithelial Cells

Overview

Journal Mol Pharmacol

Specialties Molecular Biology
Pharmacology

Date 1997 Mar 1

PMID 9058608

Citations 4

Authors

V Casavola

L Guerra

S J Reshkin

K A Jacobson

H Murer

Affiliations

Soon will be listed here.

Abstract

The effect of adenosine on Na+/H+ exchange activity was examined in cultured A6 renal epithelial cells. Adenosine and its analogue N6-cyclopentyladenosine (CPA) had different effects on Na+/H+ exchange activity depending on the side of addition. Basolateral CPA induced a stimulation of Na+/H+ exchange activity that was completely prevented by preincubation with an A2A-selective antagonist, 8-(3-chlorostyryl)caffeine, whereas apical CPA induced a slight but significant inhibition of Na+/H+ exchange activity that was significantly reduced by the A1-receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine. Protein kinase C activation may be involved in mediating the apical CPA inhibition of Na+/H+ exchange activity; this inhibition was prevented by the protein kinase C inhibitor calphostin C. Treatment with either forskolin or 8-bromo-cAMP significantly stimulated Na+/H+ exchange activity; only basolateral CPA addition induced an increase in cAMP level. These observations together with the finding that the CPA-dependent stimulation of exchange activity was prevented by the protein kinase A inhibitor H-89 support the hypothesis that basolateral CPA stimulates Na+/H+ exchange via adenylate cyclase/protein kinase A activation. Basolateral CPA also increased transepithelial Na+ transport, and this stimulation was prevented by the Na+/H+ exchange inhibitor HOE-694, suggesting that changes in pHi during hormone action can act as an intermediate in the second-messenger cascade.

Citing Articles

Stimulation of Xenopus P2Y1 receptor activates CFTR in A6 cells.

Guerra L, Favia M, Fanelli T, Calamita G, Svetlo M, Bagorda A Pflugers Arch. 2004; 449(1):66-75.

PMID: 15235914 DOI: 10.1007/s00424-004-1293-2.

Purinergic regulation of epithelial transport.

Bucheimer R, Linden J J Physiol. 2003; 555(Pt 2):311-21.

PMID: 14694149 PMC: 1664845. DOI: 10.1113/jphysiol.2003.056697.

Molecular aspects of acute inhibition of Na(+)-H(+) exchanger NHE3 by A(2)-adenosine receptor agonists.

Di Sole F, Cerull R, Casavola V, Moe O, Burckhardt G, Helmle-Kolb C J Physiol. 2002; 541(Pt 2):529-43.

PMID: 12042357 PMC: 2290320. DOI: 10.1113/jphysiol.2001.013438.

Adenosine inhibits the transfected Na+-H+ exchanger NHE3 in Xenopus laevis renal epithelial cells (A6/C1).

Di Sole F, Casavola V, Mastroberardino L, Verrey F, Moe O, Burckhardt G J Physiol. 1999; 515 ( Pt 3):829-42.

PMID: 10066908 PMC: 2269197. DOI: 10.1111/j.1469-7793.1999.829ab.x.

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