Identification of ATP-dependent Phosphofructokinase As a Regulatory Step in the Glycolytic Pathway of the Actinomycete Streptomyces Coelicolor A3(2)

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1997 Mar 1

PMID 9055413

Citations 9

Authors

A M Alves

G J Euverink

M J Bibb

L Dijkhuizen

Affiliations

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Abstract

The ATP-dependent phosphofructokinase (ATP-PFK) of Streptomyces coelicolor A3(2) was purified to homogeneity (1,600-fold) and characterized (110 kDa, with a single type of subunit of 40 kDa); it is allosterically inhibited by phosphoenolpyruvate. Cloning of the pfk gene of S. coelicolor A3(2) and analysis of the deduced amino acid sequence (343 amino acids; 36,667 Da) revealed high similarities to the PPi-PFK enzyme from Amycolatopsis methanolica (tetramer, nonallosteric; 70%) and to the allosteric ATP-PFK enzymes from other bacteria, e.g., Escherichia coli (tetramer; 37%) and Bacillus stearothermophilus (tetramer, 41%). Further structural and functional analysis of the two actinomycete PFK enzymes should elucidate the features of these proteins that determine substrate specificity (ATP versus PPi) and allosteric (in)sensitivity.

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References

Reeves R, South D, Blytt H, WARREN L . Pyrophosphate:D-fructose 6-phosphate 1-phosphotransferase. A new enzyme with the glycolytic function of 6-phosphofructokinase. J Biol Chem. 1974; 249(24):7737-41. View

Currie P, Sullivan D . Structure and expression of the gene encoding phosphofructokinase (PFK) in Drosophila melanogaster. J Biol Chem. 1994; 269(40):24679-87. View

Kotlarz D, Buc H . Two Escherichia coli fructose-6-phosphate kinases. Preparative purification, oligomeric structure and immunological studies. Biochim Biophys Acta. 1977; 484(1):35-48. DOI: 10.1016/0005-2744(77)90111-5. View

Uyeda K . Phosphofructokinase. Adv Enzymol Relat Areas Mol Biol. 1979; 48:193-244. DOI: 10.1002/9780470122938.ch4. View

Alves A, Euverink G, Hektor H, Hessels G, van der Vlag J, Vrijbloed J . Enzymes of glucose and methanol metabolism in the actinomycete Amycolatopsis methanolica. J Bacteriol. 1994; 176(22):6827-35. PMC: 197050. DOI: 10.1128/jb.176.22.6827-6835.1994. View

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

Huang M, Albach R, Chang K, Tripathi R, KEMP R . Cloning and sequencing a putative pyrophosphate-dependent phosphofructokinase gene from Entamoeba histolytica. Biochim Biophys Acta. 1995; 1260(2):215-7. DOI: 10.1016/0167-4781(94)00216-p. View

Rozario C, Smith M, Muller M . Primary sequence of a putative pyrophosphate-linked phosphofructokinase gene of Giardia lamblia. Biochim Biophys Acta. 1995; 1260(2):218-22. DOI: 10.1016/0167-4781(94)00217-q. View

Wessberg K, Skolnick S, Xu J, Marciano-Cabral F, KEMP R . Cloning, sequencing and expression of the pyrophosphate-dependent phosphofructo-1-kinase from Naegleria fowleri. Biochem J. 1995; 307 ( Pt 1):143-9. PMC: 1136756. DOI: 10.1042/bj3070143. View

10.

Takahashi N, Kalfas S, Yamada T . Phosphorylating enzymes involved in glucose fermentation of Actinomyces naeslundii. J Bacteriol. 1995; 177(20):5806-11. PMC: 177402. DOI: 10.1128/jb.177.20.5806-5811.1995. View

11.

Alves A, Meijer W, Vrijbloed J, Dijkhuizen L . Characterization and phylogeny of the pfp gene of Amycolatopsis methanolica encoding PPi-dependent phosphofructokinase. J Bacteriol. 1996; 178(1):149-55. PMC: 177632. DOI: 10.1128/jb.178.1.149-155.1996. View

12.

Redenbach M, Kieser H, Denapaite D, Eichner A, Cullum J, Kinashi H . A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol. 1996; 21(1):77-96. DOI: 10.1046/j.1365-2958.1996.6191336.x. View

13.

Henikoff S . Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984; 28(3):351-9. DOI: 10.1016/0378-1119(84)90153-7. View

14.

Hellinga H, Evans P . Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase. Eur J Biochem. 1985; 149(2):363-73. DOI: 10.1111/j.1432-1033.1985.tb08934.x. View

15.

Heinisch J . Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast. Mol Gen Genet. 1986; 202(1):75-82. DOI: 10.1007/BF00330520. View

16.

Poolman B, Smid E, Veldkamp H, Konings W . Bioenergetic consequences of lactose starvation for continuously cultured Streptococcus cremoris. J Bacteriol. 1987; 169(4):1460-8. PMC: 211968. DOI: 10.1128/jb.169.4.1460-1468.1987. View

17.

Lee C, Kao M, French B, Putney S, Chang S . The rabbit muscle phosphofructokinase gene. Implications for protein structure, function, and tissue specificity. J Biol Chem. 1987; 262(9):4195-9. View

18.

Nakajima H, Noguchi T, Yamasaki T, Kono N, Tanaka T, Tarui S . Cloning of human muscle phosphofructokinase cDNA. FEBS Lett. 1987; 223(1):113-6. DOI: 10.1016/0014-5793(87)80519-7. View

19.

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

20.

Valdez B, Chang S, YOUNATHAN E . Site-directed mutagenesis at the regulatory site of fructose 6-phosphate-1-kinase from Bacillus stearothermophilus. Biochem Biophys Res Commun. 1988; 156(1):537-42. DOI: 10.1016/s0006-291x(88)80875-1. View