Mg2+/Ca(2+)-ATPase Activity is Not Enriched in Synaptic Vesicles Isolated from Rat Cerebral Cortex

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1997 Mar 1

PMID 9051664

Authors

G Rodriguez de Lores Arnaiz

A PELLEGRINO DE IRALDI

Affiliations

Soon will be listed here.

Abstract

Neuronal ATPases comprise a wide variety of enzymes which are not uniformly distributed in different membrane preparations. Since purified vesicle fractions have Mg2+/Ca(2+)-ATPase, the purpose of the present study was to know whether such enzyme activities have a preferential concentration in a synaptic vesicle fraction in order to be used as markers for these organelles. Resorting to a procedure developed in this Institute, we fractionated the rat cerebral cortex by differential centrifugation following osmotic shock of a crude mitochondrial fraction and separated a purified synaptic vesicle fraction over discontinuous sucrose gradients. Mg2+/Ca(2+)-ATPase activities and ultrastructural studies of isolated fractions were carried out. It was observed that similar specific activities for Mg2+/Ca(2+)-ATPases were found in all fractions studied which contain synaptic vesicles and/or membranes. Although the present results confirm the presence of Mg2+ and Ca(2+)-ATPase activities in synaptic vesicles preparations, they do not favor the contention that Mg2+/Ca(2+)-ATPase is a good marker for synaptic vesicles.

References

Benzi G, Gorini A, Arnaboldi R, Ghigini B, Villa R . Age-related alterations by chronic intermittent hypoxia on cerebral synaptosomal ATPase activities. J Neural Transm Suppl. 1994; 44:159-71. DOI: 10.1007/978-3-7091-9350-1_12. View

Hicks B, Parsons S . Characterization of the P-type and V-type ATPases of cholinergic synaptic vesicles and coupling of nucleotide hydrolysis to acetylcholine transport. J Neurochem. 1992; 58(4):1211-20. DOI: 10.1111/j.1471-4159.1992.tb11331.x. View

Kortje K, Freihofer D, Rahmann H . Cytochemical localization of high-affinity Ca2(+)-ATPase activity in synaptic terminals. J Histochem Cytochem. 1990; 38(6):895-900. DOI: 10.1177/38.6.2139888. View

Rodriguez De Lores A, Alberici M, De Robertis E . Ultrastructural and enzymic studies of cholinergic and non-cholinergic synaptic membranes isolated from brain cortex. J Neurochem. 1967; 14(2):215-25. DOI: 10.1111/j.1471-4159.1967.tb05897.x. View

Mata M, Fink D . Ca++-ATPase in the central nervous system: an EM cytochemical study. J Histochem Cytochem. 1989; 37(7):971-80. DOI: 10.1177/37.7.2525142. View

Zinchuk V, Tushevsky V, Bulavka A . Enzyme ultracytochemical demonstration of Ca(++)-ATPase in the rat cerebral cortex. Folia Histochem Cytobiol. 1992; 30(1):13-6. View

Albers R, RODRIGUEZDE LORES , DeROBERTIS E . SODIUM-POTASSIUM-ACTIVATED ATPASE AND POTASSIUM-ACTIVATED P-NITROPHENYLPHOSPHATASE: A COMPARISON OF THEIR SUBCELLULAR LOCALIZATIONS IN RAT BRAIN. Proc Natl Acad Sci U S A. 1965; 53:557-64. PMC: 336976. DOI: 10.1073/pnas.53.3.557. View

LOWRY O, Lopez J . The determination of inorganic phosphate in the presence of labile phosphate esters. J Biol Chem. 2010; 162:421-8. View

De Robertis E, Rodriguez de Lores Arnaiz G, Salganicoff L, PELLEGRINO DE IRALDI A, Zieher L . Isolation of synaptic vesicles and structural organization of the acetycholine system within brain nerve endings. J Neurochem. 1963; 10:225-35. DOI: 10.1111/j.1471-4159.1963.tb05038.x. View

10.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

11.

De Robertis E, Rodriguez de Lores Arnaiz G, PELLEGRINO DE IRALDI A . Isolation of synaptic vesicles from nerve endings of the rat brain. Nature. 1962; 194:794-5. DOI: 10.1038/194794a0. View

12.

Rodriguez de Lores Arnaiz G . In search of synaptosomal Na+,K(+)-ATPase regulators. Mol Neurobiol. 1992; 6(4):359-75. DOI: 10.1007/BF02757941. View

13.

PELLEGRINO DE IRALDI A, de Lores Arnaiz G . Thiamine pyrophosphatase activity in a fraction rich in golgi-like structures from crushed sciatic nerve of the cat. J Neurochem. 1970; 17(11):1601-6. DOI: 10.1111/j.1471-4159.1970.tb03730.x. View

14.

Benzi G, Gorini A, Ghigini B, Arnaboldi R, Villa R . Modifications by hypoxia and drug treatment of cerebral ATPase plasticity. Neurochem Res. 1994; 19(4):517-24. DOI: 10.1007/BF00967332. View