Development of a Nested PCR Assay for Detection of Feline Infectious Peritonitis Virus in Clinical Specimens

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1997 Mar 1

PMID 9041410

Citations 19

Authors

D A Gamble

A Lobbiani

M Gramegna

L E Moore

G Colucci

Affiliations

Soon will be listed here.

Abstract

A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infectious peritonitis (FIP). The target sequence for the assay is in the S1 region of the peplomer protein E2 gene. A vaccine strain of FIPV and two wild-type FIPV strains tested positive, but FECV, TGEV, and CCV tested negative. Preliminary tests with 12 cats with clinical evidence of effusive FIP and 11 cats with an illness associated with effusions, but attributed to other causes, were performed. Eleven of the 12 cats with effusive FIP tested positive, while 1 was negative. Ten of the 11 cats ill from other causes tested negative, while 1 was positive. On the basis of clinical laboratory and histopathologic criteria, the preliminary sensitivity and specificity of the assay were 91.6 and 94%, respectively.

Citing Articles

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Usefulness of acute phase proteins in differentiating between feline infectious peritonitis and other diseases in cats with body cavity effusions.

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References

BOHL E, Gupta R, Olquin M, Saif L . Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect Immun. 1972; 6(3):289-301. PMC: 422530. DOI: 10.1128/iai.6.3.289-301.1972. View

Keenan K, JERVIS H, MARCHWICKI R, Binn L . Intestinal infection of neonatal dogs with canine coronavirus 1-71: studies by virologic, histologic, histochemical, and immunofluorescent techniques. Am J Vet Res. 1976; 37(3):247-56. View

Pedersen N, Ward J, Mengeling W . Antigenic relationship of the feline infectious peritonitis virus to coronaviruses of other species. Arch Virol. 1978; 58(1):45-53. PMC: 7087319. DOI: 10.1007/BF01315534. View

Horzinek M, Osterhaus A . The virology and pathogenesis of feline infectious peritonitis. Brief review. Arch Virol. 1979; 59(1-2):1-15. PMC: 7087126. DOI: 10.1007/BF01317889. View

Evermann J, Baumgartener L, OTT R, DAVIS E, McKeirnan A . Characterization of a feline infectious peritonitis virus isolate. Vet Pathol. 1981; 18(2):256-65. DOI: 10.1177/030098588101800214. View

Pedersen N, Boyle J, Floyd K, Fudge A, Barker J . An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis. Am J Vet Res. 1981; 42(3):368-77. View

Herrewegh A, de Groot R, Cepica A, Egberink H, Horzinek M, Rottier P . Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR. J Clin Microbiol. 1995; 33(3):684-9. PMC: 228014. DOI: 10.1128/jcm.33.3.684-689.1995. View

Gerstman B, Cappucci D . Evaluating the reliability of diagnostic test results. J Am Vet Med Assoc. 1986; 188(3):248-51. View

Fiscus S, Teramoto Y . Antigenic comparison of feline coronavirus isolates: evidence for markedly different peplomer glycoproteins. J Virol. 1987; 61(8):2607-13. PMC: 255709. DOI: 10.1128/JVI.61.8.2607-2613.1987. View

10.

de Groot R, Maduro J, Lenstra J, Horzinek M, van der Zeijst B, Spaan W . cDNA cloning and sequence analysis of the gene encoding the peplomer protein of feline infectious peritonitis virus. J Gen Virol. 1987; 68 ( Pt 10):2639-46. DOI: 10.1099/0022-1317-68-10-2639. View

11.

Pedersen N . Virologic and immunologic aspects of feline infectious peritonitis virus infection. Adv Exp Med Biol. 1987; 218:529-50. DOI: 10.1007/978-1-4684-1280-2_69. View

12.

Rosenberg E, Keiser J, Gross J, Ross A, Nasim S, Garrett C . Nested polymerase chain reaction assay for the detection of cytomegalovirus overcomes false positives caused by contamination with fragmented DNA. J Med Virol. 1990; 30(2):85-91. DOI: 10.1002/jmv.1890300202. View

13.

Erlich H, GELFAND D, Sninsky J . Recent advances in the polymerase chain reaction. Science. 1991; 252(5013):1643-51. DOI: 10.1126/science.2047872. View

14.

Pedersen N, Evermann J, McKeirnan A, OTT R . Pathogenicity studies of feline coronavirus isolates 79-1146 and 79-1683. Am J Vet Res. 1984; 45(12):2580-5. View