Noradrenergic Hyperinnervation May Inhibit Necrosis of Coronary Arterial Smooth Muscle Cells in Stroke-prone Spontaneously Hypertensive Rats

Overview

Journal Virchows Arch

Specialties Cell Biology
Molecular Biology
Pathology

Date 1997 Jan 1

PMID 9037318

Citations 1

Authors

M Kondo

T Fujiwara

T Miyazaki

M Terade

R TABEI

Affiliations

Soon will be listed here.

Abstract

Noradrenergic (NA) nerve fibre distribution and vascular smooth muscle morphology were investigated in the coronary artery of stroke-prone spontaneously hypertensive rats (SHRSP). Fluorescent NA nerve fibres of SHRSP aged 10, 30, 60, 90 and 180 days were examined by the glyoxylic acid method and compared with those of age-matched normotensive Wistar Kyoto (WKY) rats. The distribution densities of NA nerve fibres were measured by quantitative image analysis using the Interactive Bildanalyse System. The densities of NA nerve fibres of the left coronary artery of SHRSP were significantly higher than those of WKY rats at all ages examined. NA hyperinnervation in the coronary artery of SHRSP may be caused by the hyperfunction of the stellate ganglia which innervate the coronary arteries. Scanning electron microscopy observations showed that the surface of smooth muscle cells of the left coronary artery in SHRSP was smooth and similar to that of WKY rats at 120 days of age, but was slightly modified by more invaginations and projections than that in WKY rats at 180 days of age. No necrotic cells, however, were found in SHRSP. By transmission electron microscopy the smooth muscle cells in SHRSP were shown to be irregular in profile with deep indentations of the plasma membrane and surrounded by many layers of basal laminalike material, but no necrotic cells were found. We suggest that NA hyperinnervation protects the vascular smooth muscle cells from necrosis in the coronary artery of SHRSP by a trophic effect mediated by NA nerve fibres.

Citing Articles

Scanning and transmission electron microscopic observation of femoral head feeding vessels in stroke-prone spontaneously hypertensive rats.

Amemiya M, Yashiro T, Kikuchi M, Kouki T, Nakama S, Hoshino Y Med Mol Morphol. 2011; 44(3):139-45.

PMID: 21922385 DOI: 10.1007/s00795-010-0518-z.

References

Kondo M, Terada M, Shimizu D, Fujiwara T, TABEI R . Morphometric study of the superior cervical and stellate ganglia of spontaneously hypertensive rats during the prehypertensive stage. Virchows Arch B Cell Pathol Incl Mol Pathol. 1990; 58(5):371-6. DOI: 10.1007/BF02890094. View

Baumbach G, Heistad D . Adaptive changes in cerebral blood vessels during chronic hypertension. J Hypertens. 1991; 9(11):987-91. DOI: 10.1097/00004872-199111000-00002. View

Baumbach G, Dobrin P, Hart M, Heistad D . Mechanics of cerebral arterioles in hypertensive rats. Circ Res. 1988; 62(1):56-64. DOI: 10.1161/01.res.62.1.56. View

Fujiwara T, Uehara Y . The cytoarchitecture of the medial layer in rat thoracic aorta: a scanning electron-microscopic study. Cell Tissue Res. 1992; 270(1):165-72. DOI: 10.1007/BF00381891. View

Kondo M . Autoradiographic study of 3H-DOPA uptake by superior cervical and stellate ganglia of spontaneously hypertensive rats during the prehypertensive stage. Virchows Arch B Cell Pathol Incl Mol Pathol. 1987; 54(3):190-3. DOI: 10.1007/BF02899211. View

Clozel J, Kuhn H, Hefti F . Decreases of vascular hypertrophy in four different types of arteries in spontaneously hypertensive rats. Am J Med. 1989; 87(6B):92S-95S. DOI: 10.1016/0002-9343(89)90102-2. View

Johannsen U, Mark A, Marcus M . Responsiveness to cardiac sympathetic nerve stimulation during maximal coronary dilation produced by adenosine. Circ Res. 1982; 50(4):510-7. DOI: 10.1161/01.res.50.4.510. View

Amenta F, Bronzetti E, Cavallotti C, Felici L . Quantitative image analysis of the density and pattern of adrenergic innervation of blood vessels of rat spinal cord. J Auton Nerv Syst. 1987; 18(3):261-4. DOI: 10.1016/0165-1838(87)90124-x. View

Mano M, Jeffreson S, Head R . Vascular, ganglia and cardiac catecholamine disposition in the spontaneously hypertensive rat and in the stroke-prone spontaneously hypertensive rat. J Vasc Res. 1992; 29(1):8-12. DOI: 10.1159/000158925. View

10.

Kristek F, Gerova M . Autonomic nerve terminals in relation to contractile and non-contractile structures in the conduit coronary artery of the dog. Acta Anat (Basel). 1987; 129(2):149-54. DOI: 10.1159/000146391. View

11.

Chilian W, Layne S, Eastham C, Marcus M . Heterogeneous microvascular coronary alpha-adrenergic vasoconstriction. Circ Res. 1989; 64(2):376-88. DOI: 10.1161/01.res.64.2.376. View

12.

Donohue S, Stitzel R, Head R . Time course of changes in the norepinephrine content of tissues from spontaneously hypertensive and Wistar Kyoto rats. J Pharmacol Exp Ther. 1988; 245(1):24-31. View

13.

Sadoshima S, Busija D, Heistad D . Mechanisms of protection against stroke in stroke-prone spontaneously hypertensive rats. Am J Physiol. 1983; 244(3):H406-12. DOI: 10.1152/ajpheart.1983.244.3.H406. View

14.

Warshaw D, Mulvany M, Halpern W . Mechanical and morphological properties of arterial resistance vessels in young and old spontaneously hypertensive rats. Circ Res. 1979; 45(2):250-9. DOI: 10.1161/01.res.45.2.250. View

15.

Miller B, Connors B, Bohlen H, Evan A . Cell and wall morphology of intestinal arterioles from 4- to 6- and 17- to 19-week-old Wistar-Kyoto and spontaneously hypertensive rats. Hypertension. 1987; 9(1):59-68. DOI: 10.1161/01.hyp.9.1.59. View

16.

Vatner S, Pagani M, Manders W, Pasipoularides A . Alpha adrenergic vasoconstriction and nitroglycerin vasodilation of large coronary arteries in the conscious dog. J Clin Invest. 1980; 65(1):5-14. PMC: 371334. DOI: 10.1172/JCI109659. View

17.

Kondo M . Autoradiographic study of 3H-lysine uptake by superior cervical and stellate ganglia in prehypertensive spontaneously hypertensive rats. Virchows Arch B Cell Pathol Incl Mol Pathol. 1986; 52(4):299-304. DOI: 10.1007/BF02889972. View

18.

Young M, Vatner S . Regulation of large coronary arteries. Circ Res. 1986; 59(6):579-96. DOI: 10.1161/01.res.59.6.579. View

19.

Kawai Y, Ohhashi T . Histochemical studies of the adrenergic innervation of canine cerebral arteries. J Auton Nerv Syst. 1986; 15(2):103-8. DOI: 10.1016/0165-1838(86)90007-x. View

20.

Head R, Cassis L, Robinson R, Westfall D, Stitzel R . Altered catecholamine contents in vascular and nonvascular tissues in genetically hypertensive rats. Blood Vessels. 1985; 22(4):196-204. DOI: 10.1159/000158601. View