In Vitro Transcription of Human T-cell Leukemia Virus Type 1 is RNA Polymerase II Dependent

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1997 Mar 1

PMID 9032404

Citations 6

Authors

B A Lenzmeier

J K Nyborg

Affiliations

Soon will be listed here.

Abstract

The HTLV-1 promoter directs RNA polymerase II transcription of viral genomic RNA in vivo. However, it has been reported that in vitro, a unique RNA polymerase, with characteristics of RNA polymerases II and III, is capable of HTLV-1 transcription (G. Piras, F. Kashanchi, M. F. Radonovich, J. F. Duvall, and J. N. Brady, J. Virol. 68:6170-6179, 1994). To further characterize the polymerase involved in HTLV-1 transcription in vitro, runoff transcription assays were performed with a variety of extracts and RNA polymerase inhibitors. Under all in vitro reaction conditions tested, RNA polymerase II appeared to be the only polymerase capable of correct transcriptional initiation from the HTLV-1 promoter. Synthesis of the specific HTLV-1 RNA transcript showed sensitivities to the RNA polymerase inhibitors tagetitoxin and alpha-amanitin that are consistent with RNA polymerase II transcription. Together, these data indicate that in vitro, as in vivo, the HTLV-1 promoter directs transcription by RNA polymerase II.

Citing Articles

Prediction and analysis of essential genes using the enrichments of gene ontology and KEGG pathways.

Chen L, Zhang Y, Wang S, Zhang Y, Huang T, Cai Y PLoS One. 2017; 12(9):e0184129.

PMID: 28873455 PMC: 5584762. DOI: 10.1371/journal.pone.0184129.

RNA polymerase III accurately initiates transcription from RNA polymerase II promoters in vitro.

Duttke S J Biol Chem. 2014; 289(29):20396-404.

PMID: 24917680 PMC: 4106352. DOI: 10.1074/jbc.M114.563254.

Activator-dependent p300 acetylation of chromatin in vitro: enhancement of transcription by disruption of repressive nucleosome-nucleosome interactions.

Szerlong H, Prenni J, Nyborg J, Hansen J J Biol Chem. 2010; 285(42):31954-64.

PMID: 20720004 PMC: 2952196. DOI: 10.1074/jbc.M110.148718.

The coactivators CBP/p300 and the histone chaperone NAP1 promote transcription-independent nucleosome eviction at the HTLV-1 promoter.

Sharma N, Nyborg J Proc Natl Acad Sci U S A. 2008; 105(23):7959-63.

PMID: 18523016 PMC: 2430344. DOI: 10.1073/pnas.0800534105.

Human T-cell leukemia virus type 1 Tax requires direct access to DNA for recruitment of CREB binding protein to the viral promoter.

Lenzmeier B, Giebler H, Nyborg J Mol Cell Biol. 1998; 18(2):721-31.

PMID: 9447968 PMC: 108783. DOI: 10.1128/MCB.18.2.721.

References

Jacob S, Sajdel E, MUNRO H . Specific action of alpha-amanitin on mammalian RNA polymerase protein. Nature. 1970; 225(5227):60-2. DOI: 10.1038/225060b0. View

Yin M, Paulssen E, Seeler J, Gaynor R . Protein domains involved in both in vivo and in vitro interactions between human T-cell leukemia virus type I tax and CREB. J Virol. 1995; 69(6):3420-32. PMC: 189054. DOI: 10.1128/JVI.69.6.3420-3432.1995. View

Chambon P . Eukaryotic nuclear RNA polymerases. Annu Rev Biochem. 1975; 44:613-38. DOI: 10.1146/annurev.bi.44.070175.003145. View

Duvall J, Kashanchi F, Cvekl A, Radonovich M, Piras G, Brady J . Transactivation of the human T-cell lymphotropic virus type 1 Tax1-responsive 21-base-pair repeats requires Holo-TFIID and TFIIA. J Virol. 1995; 69(8):5077-86. PMC: 189325. DOI: 10.1128/JVI.69.8.5077-5086.1995. View

Zhao L, Giam C . Interaction of the human T-cell lymphotrophic virus type I (HTLV-I) transcriptional activator Tax with cellular factors that bind specifically to the 21-base-pair repeats in the HTLV-I enhancer. Proc Natl Acad Sci U S A. 1991; 88(24):11445-9. PMC: 53152. DOI: 10.1073/pnas.88.24.11445. View

Hoeffler J, Lustbader J, Chen C . Identification of multiple nuclear factors that interact with cyclic adenosine 3',5'-monophosphate response element-binding protein and activating transcription factor-2 by protein-protein interactions. Mol Endocrinol. 1991; 5(2):256-66. DOI: 10.1210/mend-5-2-256. View

Matthews M, Markowitz R, Dynan W . In vitro activation of transcription by the human T-cell leukemia virus type I Tax protein. Mol Cell Biol. 1992; 12(5):1986-96. PMC: 364369. DOI: 10.1128/mcb.12.5.1986-1996.1992. View

Johnson D, Fan R, Treinies M . Analysis of the molecular mechanisms for the species-specific transcription of Drosophila and human tRNA gene transcription components. J Biol Chem. 1991; 266(24):16037-43. View

Dynan W, Tjian R . Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II. Cell. 1983; 32(3):669-80. DOI: 10.1016/0092-8674(83)90053-3. View

10.

Brauweiler A, Garl P, Franklin A, Giebler H, Nyborg J . A molecular mechanism for human T-cell leukemia virus latency and Tax transactivation. J Biol Chem. 1995; 270(21):12814-22. DOI: 10.1074/jbc.270.21.12814. View

11.

Gottesfeld J, Johnson D, Nyborg J . Transcriptional activation of RNA polymerase III-dependent genes by the human T-cell leukemia virus type 1 tax protein. Mol Cell Biol. 1996; 16(4):1777-85. PMC: 231164. DOI: 10.1128/MCB.16.4.1777. View

12.

Niki M, Ohtani K, Nakamura M, Sugamura K . Multistep regulation of enhancer activity of the 21-base-pair element of human T-cell leukemia virus type I. J Virol. 1992; 66(7):4348-57. PMC: 241241. DOI: 10.1128/JVI.66.7.4348-4357.1992. View

13.

Franklin A, Nyborg J . Mechanisms of Tax Regulation of Human T Cell Leukemia Virus Type I Gene Expression. J Biomed Sci. 1995; 2(1):17-29. DOI: 10.1007/BF02257921. View

14.

Brady J, Jeang K, Duvall J, Khoury G . Identification of p40x-responsive regulatory sequences within the human T-cell leukemia virus type I long terminal repeat. J Virol. 1987; 61(7):2175-81. PMC: 254240. DOI: 10.1128/JVI.61.7.2175-2181.1987. View

15.

Zhao L, Giam C . Human T-cell lymphotropic virus type I (HTLV-I) transcriptional activator, Tax, enhances CREB binding to HTLV-I 21-base-pair repeats by protein-protein interaction. Proc Natl Acad Sci U S A. 1992; 89(15):7070-4. PMC: 49647. DOI: 10.1073/pnas.89.15.7070. View

16.

Suzuki T, Fujisawa J, Toita M, Yoshida M . The trans-activator tax of human T-cell leukemia virus type 1 (HTLV-1) interacts with cAMP-responsive element (CRE) binding and CRE modulator proteins that bind to the 21-base-pair enhancer of HTLV-1. Proc Natl Acad Sci U S A. 1993; 90(2):610-4. PMC: 45713. DOI: 10.1073/pnas.90.2.610. View

17.

Bantignies F, Rousset R, Desbois C, Jalinot P . Genetic characterization of transactivation of the human T-cell leukemia virus type 1 promoter: Binding of Tax to Tax-responsive element 1 is mediated by the cyclic AMP-responsive members of the CREB/ATF family of transcription factors. Mol Cell Biol. 1996; 16(5):2174-82. PMC: 231205. DOI: 10.1128/MCB.16.5.2174. View

18.

Franklin A, Kubik M, Uittenbogaard M, Brauweiler A, Utaisincharoen P, Matthews M . Transactivation by the human T-cell leukemia virus Tax protein is mediated through enhanced binding of activating transcription factor-2 (ATF-2) ATF-2 response and cAMP element-binding protein (CREB). J Biol Chem. 1993; 268(28):21225-31. View

19.

Beimling P, Moelling K . Direct interaction of CREB protein with 21 bp Tax-response elements of HTLV-ILTR. Oncogene. 1992; 7(2):257-62. View

20.

Dingermann T, Sharp S, Schaack J, Soll D . Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions. J Biol Chem. 1983; 258(17):10395-402. View