Activated Levels of RRNA Synthesis in Fission Yeast Are Driven by an Intergenic RDNA Region Positioned over 2500 Nucleotides Upstream of the Initiation Site

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1997 Feb 1

PMID 9016610

Citations 6

Authors

Z Liu

A Zhao

L Chen

L Pape

Affiliations

Soon will be listed here.

Abstract

RNA polymerase I-catalyzed synthesis of the Schizosaccharomyces pombe approximately 37S pre-rRNAs was shown to be sensitive to regulatory sequences located several kilobases upstream of the initiation site for the rRNA gene. An in vitro transcription system for RNA polymerase I-catalyzed RNA synthesis was established that supports correct and activated transcription from templates bearing a full S. pombe rRNA gene promoter. A 780 bp region starting at -2560 significantly stimulates transcription of ac is-located rDNA promoter and competes with an rDNA promoter in trans, thus displaying some of the activities of rDNA transcriptional enhancers in vitro. Deletion of a 30 bp enhancer-homologous domain in this 780 bp far upstream region blocked its cis-stimulatory effect. The sequence of the S. pombe 3.5 kb intergenic spacer was determined and its organization differs from that of vertebrate, Drosophila, Acanthamoeba and plant intergenic rDNA spacers: it does not contain multiple, imperfect copies of the rRNA gene promoter nor repetitive elements of 140 bp, as are found in vertebrate rDNA enhancers.

Citing Articles

Characterization of the fission yeast ribosomal DNA binding factor: components share homology with Upstream Activating Factor and with SWI/SNF subunits.

Liu M, Guo A, Boukhgalter B, Van Den Heuvel K, Tripp M, Pape L Nucleic Acids Res. 2002; 30(24):5347-59.

PMID: 12490702 PMC: 140063. DOI: 10.1093/nar/gkf683.

Identification and characterization of transcription factor IIIA from Schizosaccharomyces pombe.

Schulman D, Setzer D Nucleic Acids Res. 2002; 30(13):2772-81.

PMID: 12087160 PMC: 117040. DOI: 10.1093/nar/gkf385.

Faithful in vitro transcription by fission yeast RNA polymerase III reveals unique alpha-amanitin sensitivity.

Rodicker F, Ossenbuhl F, Michels D, Benecke B Gene Expr. 2000; 8(3):165-74.

PMID: 10634318 PMC: 6157371.

Multiple orientation-dependent, synergistically interacting, similar domains in the ribosomal DNA replication origin of the fission yeast, Schizosaccharomyces pombe.

Kim S, Huberman J Mol Cell Biol. 1998; 18(12):7294-303.

PMID: 9819416 PMC: 109311. DOI: 10.1128/MCB.18.12.7294.

An immunoaffinity purified Schizosaccharomyces pombe TBP-containing complex directs correct initiation of the S.pombe rRNA gene promoter.

Chen L, Guo A, Pape L Nucleic Acids Res. 1997; 25(8):1633-40.

PMID: 9092673 PMC: 146630. DOI: 10.1093/nar/25.8.1633.

References

Ge H, Roeder R . Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes. Cell. 1994; 78(3):513-23. DOI: 10.1016/0092-8674(94)90428-6. View

Putnam C, Copenhaver G, Denton M, Pikaard C . The RNA polymerase I transactivator upstream binding factor requires its dimerization domain and high-mobility-group (HMG) box 1 to bend, wrap, and positively supercoil enhancer DNA. Mol Cell Biol. 1994; 14(10):6476-88. PMC: 359177. DOI: 10.1128/mcb.14.10.6476-6488.1994. View

Yang Q, Zwick M, Paule M . Sequence organization of the Acanthamoeba rRNA intergenic spacer: identification of transcriptional enhancers. Nucleic Acids Res. 1994; 22(22):4798-805. PMC: 308533. DOI: 10.1093/nar/22.22.4798. View

Cavanaugh A, Hempel W, Taylor L, Rogalsky V, Todorov G, Rothblum L . Activity of RNA polymerase I transcription factor UBF blocked by Rb gene product. Nature. 1995; 374(6518):177-80. DOI: 10.1038/374177a0. View

Shykind B, Kim J, Sharp P . Activation of the TFIID-TFIIA complex with HMG-2. Genes Dev. 1995; 9(11):1354-65. DOI: 10.1101/gad.9.11.1354. View

Paalman M, Henderson S . Stimulation of the mouse rRNA gene promoter by a distal spacer promoter. Mol Cell Biol. 1995; 15(8):4648-56. PMC: 230706. DOI: 10.1128/MCB.15.8.4648. View

Lopata M, Cleveland D . Surprising S1-resistant trimolecular hybrids: potential complication in interpretation of S1 mapping analyses. Mol Cell Biol. 1985; 5(10):2842-6. PMC: 367024. DOI: 10.1128/mcb.5.10.2842-2846.1985. View

Tower J . Transcription of cloned eukaryotic ribosomal RNA genes. Annu Rev Biochem. 1986; 55:801-30. DOI: 10.1146/annurev.bi.55.070186.004101. View

Wang X, Calame K . SV40 enhancer-binding factors are required at the establishment but not the maintenance step of enhancer-dependent transcriptional activation. Cell. 1986; 47(2):241-7. DOI: 10.1016/0092-8674(86)90446-0. View

10.

Grummt I, Kuhn A, Bartsch I, Rosenbauer H . A transcription terminator located upstream of the mouse rDNA initiation site affects rRNA synthesis. Cell. 1986; 47(6):901-11. DOI: 10.1016/0092-8674(86)90805-6. View

11.

Elion E, Warner J . An RNA polymerase I enhancer in Saccharomyces cerevisiae. Mol Cell Biol. 1986; 6(6):2089-97. PMC: 367749. DOI: 10.1128/mcb.6.6.2089-2097.1986. View

12.

Cassidy B, Rothblum L . Additional RNA polymerase I initiation site within the nontranscribed spacer region of the rat rRNA gene. Mol Cell Biol. 1987; 7(7):2388-96. PMC: 365370. DOI: 10.1128/mcb.7.7.2388-2396.1987. View

13.

De Winter R, Moss T . A complex array of sequences enhances ribosomal transcription in Xenopus laevis. J Mol Biol. 1987; 196(4):813-27. DOI: 10.1016/0022-2836(87)90407-4. View

14.

Kuhn A, Grummt I . A novel promoter in the mouse rDNA spacer is active in vivo and in vitro. EMBO J. 1987; 6(11):3487-92. PMC: 553807. DOI: 10.1002/j.1460-2075.1987.tb02673.x. View

15.

Jones R, Moreno S, Nurse P, Jones N . Expression of the SV40 promoter in fission yeast: identification and characterization of an AP-1-like factor. Cell. 1988; 53(4):659-67. DOI: 10.1016/0092-8674(88)90581-8. View

16.

Grimaldi G, Di Nocera P . Multiple repeated units in Drosophila melanogaster ribosomal DNA spacer stimulate rRNA precursor transcription. Proc Natl Acad Sci U S A. 1988; 85(15):5502-6. PMC: 281785. DOI: 10.1073/pnas.85.15.5502. View

17.

Short J, Fernandez J, Sorge J, Huse W . Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res. 1988; 16(15):7583-600. PMC: 338428. DOI: 10.1093/nar/16.15.7583. View

18.

Weintraub H . Formation of stable transcription complexes as assayed by analysis of individual templates. Proc Natl Acad Sci U S A. 1988; 85(16):5819-23. PMC: 281856. DOI: 10.1073/pnas.85.16.5819. View

19.

Bateman E, Paule M . Promoter occlusion during ribosomal RNA transcription. Cell. 1988; 54(7):985-92. DOI: 10.1016/0092-8674(88)90113-4. View

20.

Hill J, Myers A, Koerner T, Tzagoloff A . Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast. 1986; 2(3):163-7. DOI: 10.1002/yea.320020304. View