Identification of Carcinoembryonic Antigen-producing Cells Circulating in the Blood of Patients with Colorectal Carcinoma by Reverse Transcriptase Polymerase Chain Reaction

Overview

Journal Gut

Specialty Gastroenterology

Date 1996 Nov 1

PMID 9014772

Citations 18

Authors

S Jonas

S Windeatt

C Fordy

T G Allen-Mersh

Affiliations

Soon will be listed here.

Abstract

Background: Application of the reverse transcriptase polymerase chain reaction (RT-PCR) to identification of circulating tumour cells in colorectal cancer.

Aims: To assess whether circulating malignant cells in patients with colorectal liver metastasis could be identified by RT-PCR recognition of mRNA coding for the tumour marker carcinoembryonic antigen (CEA).

Patients: A total of 31 with colorectal liver metastases and 22 no-cancer controls.

Methods: Specific cDNA primers for CEA transcripts were used to apply RT-PCR to tissue biopsy specimens, colon carcinoma cell lines, and peripheral blood samples from patients with colorectal liver metastases. A strongly CEA-expressive HT115 colorectal carcinoma cell line was used to spike blood samples from no-cancer control subjects.

Results: The limit for detection of CEA cDNA by Southern blotting using HT115 cells was 50 cells per 14 ml of spiked blood. There was a significant difference (p = 0.007) in RT-PCR positive expression between patients with liver metastasis (26/31) compared with controls (5/22). There was no significant relation between the prevalence of CEA cDNA amplification and serum CEA level or metastasis volume in patients with liver metastasis.

Conclusions: This is the first study to suggest that identification of circulating colorectal cancer cells using RT-PCR for detection of CEA cDNA is feasible.

Citing Articles

Common molecular markers between circulating tumor cells and blood exosomes in colorectal cancer: a systematic and analytical review.

Vafaei S, Fattahi F, Ebrahimi M, Janani L, Shariftabrizi A, Madjd Z Cancer Manag Res. 2019; 11:8669-8698.

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The CEA-/lo colorectal cancer cell population harbors cancer stem cells and metastatic cells.

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Evaluation of prognostic significance of circulating tumor cells detection in rectal cancer patients treated with preoperative radiotherapy: prospectively collected material data.

Nesteruk D, Rutkowski A, Fabisiewicz S, Pawlak J, Siedlecki J, Fabisiewicz A Biomed Res Int. 2014; 2014:712827.

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Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma.

Qiu M, Li Z, Zhou Z, Li Y, Wang Z, Wang F J Transl Med. 2010; 8:107.

PMID: 21040522 PMC: 2989934. DOI: 10.1186/1479-5876-8-107.

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