A Novel Western Blot Test Containing Both Viral and Recombinant Proteins for Anticytomegalovirus Immunoglobulin M Detection

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1997 Feb 1

PMID 9003603

Citations 11

Authors

T Lazzarotto

G T Maine

P Dal Monte

A Ripalti

M P Landini

Affiliations

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Abstract

We devised a novel Western blot (WB) test for anti-human cytomegalovirus (HCMV) immunoglobulin M (IgM) detection which contains viral structural polypeptides, significant portions of recombinant p150 (ppUL32), and a significant portion of the most immunogenic nonstructural protein p52 (ppUL44). This new test was evaluated in latently infected blood donors, pregnant women, and transplant recipients with ongoing HCMV infection and shown to be more sensitive and specific than traditional WB and conventional enzyme immunoassay for the detection of HCMV-specific IgM.

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