Rescue of a Segmented Negative-strand RNA Virus Entirely from Cloned Complementary DNAs

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1996 Dec 24

PMID 8986823

Citations 101

Authors

A Bridgen

R M Elliott

Affiliations

Soon will be listed here.

Abstract

We provide the first report, to our knowledge, of a helper-independent system for rescuing a segmented, negative-strand RNA genome virus entirely from cloned cDNAs. Plasmids were constructed containing full-length cDNA copies of the three Bunyamwera bunyavirus RNA genome segments flanked by bacteriophage T7 promoter and hepatitis delta virus ribozyme sequences. When cells expressing both bacteriophage T7 RNA polymerase and recombinant Bunyamwera bunyavirus proteins were transfected with these plasmids, full-length antigenome RNAs were transcribed intracellularly, and these in turn were replicated and packaged into infectious bunyavirus particles. The resulting progeny virus contained specific genetic tags characteristic of the parental cDNA clones. Reassortant viruses containing two genome segments of Bunyamwera bunyavirus and one segment of Maguari bunyavirus were also produced following transfection of appropriate plasmids. This accomplishment will allow the full application of recombinant DNA technology to manipulate the bunyavirus genome.

Citing Articles

NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts.

Terhzaz S, Kerrigan D, Almire F, Szemiel A, Hughes J, Parvy J Nat Commun. 2025; 16(1):1214.

PMID: 39890788 PMC: 11785797. DOI: 10.1038/s41467-024-54809-7.

Bioluminescent and fluorescent reporter-expressing recombinant Akabane virus (AKAV): an excellent tool to dissect viral replication.

Liu J, Wang F, Zhao J, Qi Y, Chang J, Sun C Front Microbiol. 2024; 15:1458771.

PMID: 39611086 PMC: 11602509. DOI: 10.3389/fmicb.2024.1458771.

Bunyamwera Virus Infection of -Carrying Mosquitoes Reduces Density.

Lefteri D, Rainey S, Murdochy S, Sinkins S Viruses. 2024; 16(8).

PMID: 39205310 PMC: 11360823. DOI: 10.3390/v16081336.

An Introduction to Rift Valley Fever Virus.

Weber F, Bouloy M, Lozach P Methods Mol Biol. 2024; 2824:1-14.

PMID: 39039402 DOI: 10.1007/978-1-0716-3926-9_1.

Nairovirus polymerase mutations associated with the establishment of persistent infection in human cells.

Ohta K, Saka N, Nishi Y, Nishio M J Virol. 2024; 98(3):e0169823.

PMID: 38358288 PMC: 10949423. DOI: 10.1128/jvi.01698-23.

References

Pleschka S, Jaskunas R, Engelhardt O, Zurcher T, Palese P, Garcia-Sastre A . A plasmid-based reverse genetics system for influenza A virus. J Virol. 1996; 70(6):4188-92. PMC: 190316. DOI: 10.1128/JVI.70.6.4188-4192.1996. View

ENAMI M, Luytjes W, Krystal M, Palese P . Introduction of site-specific mutations into the genome of influenza virus. Proc Natl Acad Sci U S A. 1990; 87(10):3802-5. PMC: 53991. DOI: 10.1073/pnas.87.10.3802. View

Racaniello V, Baltimore D . Cloned poliovirus complementary DNA is infectious in mammalian cells. Science. 1981; 214(4523):916-9. DOI: 10.1126/science.6272391. View

Watret G, Pringle C, Elliott R . Synthesis of bunyavirus-specific proteins in a continuous cell line (XTC-2) derived from Xenopus laevis. J Gen Virol. 1985; 66 ( Pt 3):473-82. DOI: 10.1099/0022-1317-66-3-473. View

Lees J, Pringle C, Elliott R . Nucleotide sequence of the Bunyamwera virus M RNA segment: conservation of structural features in the Bunyavirus glycoprotein gene product. Virology. 1986; 148(1):1-14. DOI: 10.1016/0042-6822(86)90398-3. View

Maddon P, Dalgleish A, McDougal J, Clapham P, Weiss R, Axel R . The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell. 1986; 47(3):333-48. DOI: 10.1016/0092-8674(86)90590-8. View

Fuerst T, Niles E, Studier F, Moss B . Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A. 1986; 83(21):8122-6. PMC: 386879. DOI: 10.1073/pnas.83.21.8122. View

Elliott R . Nucleotide sequence analysis of the small (S) RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae. J Gen Virol. 1989; 70 ( Pt 5):1281-5. DOI: 10.1099/0022-1317-70-5-1281. View

Perrotta A, Been M . A pseudoknot-like structure required for efficient self-cleavage of hepatitis delta virus RNA. Nature. 1991; 350(6317):434-6. DOI: 10.1038/350434a0. View

10.

Jin H, Elliott R . Expression of functional Bunyamwera virus L protein by recombinant vaccinia viruses. J Virol. 1991; 65(8):4182-9. PMC: 248853. DOI: 10.1128/JVI.65.8.4182-4189.1991. View

11.

Patel A, Elliott R . Characterization of Bunyamwera virus defective interfering particles. J Gen Virol. 1992; 73 ( Pt 2):389-96. DOI: 10.1099/0022-1317-73-2-389. View

12.

Ball L . Cellular expression of a functional nodavirus RNA replicon from vaccinia virus vectors. J Virol. 1992; 66(4):2335-45. PMC: 289029. DOI: 10.1128/JVI.66.4.2335-2345.1992. View

13.

Jin H, Elliott R . Mutagenesis of the L protein encoded by Bunyamwera virus and production of monospecific antibodies. J Gen Virol. 1992; 73 ( Pt 9):2235-44. DOI: 10.1099/0022-1317-73-9-2235. View

14.

Nakitare G, Elliott R . Expression of the Bunyamwera virus M genome segment and intracellular localization of NSm. Virology. 1993; 195(2):511-20. DOI: 10.1006/viro.1993.1402. View

15.

Nichol S, Spiropoulou C, Morzunov S, Rollin P, Ksiazek T, Feldmann H . Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science. 1993; 262(5135):914-7. DOI: 10.1126/science.8235615. View

16.

Garcia-Sastre A, Palese P . Genetic manipulation of negative-strand RNA virus genomes. Annu Rev Microbiol. 1993; 47:765-90. DOI: 10.1146/annurev.mi.47.100193.004001. View

17.

Boyer J, Haenni A . Infectious transcripts and cDNA clones of RNA viruses. Virology. 1994; 198(2):415-26. DOI: 10.1006/viro.1994.1053. View

18.

Chalfie M, Tu Y, Euskirchen G, Ward W, Prasher D . Green fluorescent protein as a marker for gene expression. Science. 1994; 263(5148):802-5. DOI: 10.1126/science.8303295. View

19.

Dunn E, Pritlove D, Elliott R . The S RNA genome segments of Batai, Cache Valley, Guaroa, Kairi, Lumbo, Main Drain and Northway bunyaviruses: sequence determination and analysis. J Gen Virol. 1994; 75 ( Pt 3):597-608. DOI: 10.1099/0022-1317-75-3-597. View

20.

Schnell M, Mebatsion T, Conzelmann K . Infectious rabies viruses from cloned cDNA. EMBO J. 1994; 13(18):4195-203. PMC: 395346. DOI: 10.1002/j.1460-2075.1994.tb06739.x. View