Aspergillus FabM Encodes an Essential Product That is Related to Poly(A)-binding Proteins and Activates Development when Overexpressed

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1996 Dec 1

PMID 8978035

Citations 12

Authors

J F Marhoul

T H Adams

Affiliations

Soon will be listed here.

Abstract

Overexpression of several different Aspergillus nidulans developmental regulatory genes has been shown to cause inappropriate developmental activation and growth inhibition. We previously exploited this observation that induced development caused growth inhibition in designing a screen to identify other genes that could activate development when overexpressed. We identified 16 mutants in which induced expression of different random genomic DNA sequences caused growth inhibition, accumulation of mRNA corresponding to the brlA developmental regulatory locus, and in several cases sporulation. This phenotype was designated FAB for Forced expression Activation of brlA and the genes were called fabA through fabP. Here we describe one of these genes, fabM, which is predicted to encode a poly(A)-binding protein (PABP) that is constitutively expressed and is essential for viability. While it is unclear why overexpression of the fabM caused sporulation, we showed that this activity required other known early developmental regulators including brlA beta, flbA, flbB, flbC, and fluG. We propose that fabM is an example of a gene that is not only required for growth, but also has specific functions early in development that assist, developmental induction, presumably by allowing translation of specific mRNAs like brlA.

Citing Articles

HO Induces Major Phosphorylation Changes in Critical Regulators of Signal Transduction, Gene Expression, Metabolism and Developmental Networks in .

Carrasco-Navarro U, Aguirre J J Fungi (Basel). 2021; 7(8).

PMID: 34436163 PMC: 8399174. DOI: 10.3390/jof7080624.

Functions of PUF Family RNA-Binding Proteins in .

Son S, Jang S, Park H J Microbiol Biotechnol. 2021; 31(5):676-685.

PMID: 33746193 PMC: 9706018. DOI: 10.4014/jmb.2101.01011.

The Aspergillus nidulans Pbp1 homolog is required for normal sexual development and secondary metabolism.

Soukup A, Fischer G, Luo J, Keller N Fungal Genet Biol. 2017; 100:13-21.

PMID: 28089630 PMC: 5337145. DOI: 10.1016/j.fgb.2017.01.004.

The SrkA Kinase Is Part of the SakA Mitogen-Activated Protein Kinase Interactome and Regulates Stress Responses and Development in Aspergillus nidulans.

Jaimes-Arroyo R, Lara-Rojas F, Bayram O, Valerius O, Braus G, Aguirre J Eukaryot Cell. 2015; 14(5):495-510.

PMID: 25820520 PMC: 4421008. DOI: 10.1128/EC.00277-14.

Fungal genes in context: genome architecture reflects regulatory complexity and function.

Noble L, Andrianopoulos A Genome Biol Evol. 2013; 5(7):1336-52.

PMID: 23699226 PMC: 3730340. DOI: 10.1093/gbe/evt077.

References

CLUTTERBUCK A . A mutational analysis of conidial development in Aspergillus nidulans. Genetics. 1969; 63(2):317-27. PMC: 1212347. DOI: 10.1093/genetics/63.2.317. View

Stringer M, Dean R, Sewall T, Timberlake W . Rodletless, a new Aspergillus developmental mutant induced by directed gene inactivation. Genes Dev. 1991; 5(7):1161-71. DOI: 10.1101/gad.5.7.1161. View

Zimmermann C, Orr W, Leclerc R, Barnard E, Timberlake W . Molecular cloning and selection of genes regulated in Aspergillus development. Cell. 1980; 21(3):709-15. DOI: 10.1016/0092-8674(80)90434-1. View

Berse B, Dmochowska A, Skrzypek M, Weglenski P, Bates M, WEISS R . Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans. Gene. 1983; 25(1):109-17. DOI: 10.1016/0378-1119(83)90173-7. View

Sachs A, Bond M, Kornberg R . A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression. Cell. 1986; 45(6):827-35. DOI: 10.1016/0092-8674(86)90557-x. View

Boylan M, Mirabito P, Willett C, Zimmerman C, Timberlake W . Isolation and physical characterization of three essential conidiation genes from Aspergillus nidulans. Mol Cell Biol. 1987; 7(9):3113-8. PMC: 367944. DOI: 10.1128/mcb.7.9.3113-3118.1987. View

Yu J, Wieser J, Adams T . The Aspergillus FlbA RGS domain protein antagonizes G protein signaling to block proliferation and allow development. EMBO J. 1996; 15(19):5184-90. PMC: 452262. View

Pontecorvo G, Roper J, HEMMONS L, MacDonald K, BUFTON A . The genetics of Aspergillus nidulans. Adv Genet. 1953; 5:141-238. DOI: 10.1016/s0065-2660(08)60408-3. View

Sachs A, Davis R, Kornberg R . A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. Mol Cell Biol. 1987; 7(9):3268-76. PMC: 367964. DOI: 10.1128/mcb.7.9.3268-3276.1987. View

10.

Pridmore R . New and versatile cloning vectors with kanamycin-resistance marker. Gene. 1987; 56(2-3):309-12. DOI: 10.1016/0378-1119(87)90149-1. View

11.

Osmani S, Engle D, Doonan J, Morris N . Spindle formation and chromatin condensation in cells blocked at interphase by mutation of a negative cell cycle control gene. Cell. 1988; 52(2):241-51. DOI: 10.1016/0092-8674(88)90513-2. View

12.

Adams T, Boylan M, Timberlake W . brlA is necessary and sufficient to direct conidiophore development in Aspergillus nidulans. Cell. 1988; 54(3):353-62. DOI: 10.1016/0092-8674(88)90198-5. View

13.

ODonnell K, Osmani A, Osmani S, Morris N . bimA encodes a member of the tetratricopeptide repeat family of proteins and is required for the completion of mitosis in Aspergillus nidulans. J Cell Sci. 1991; 99 ( Pt 4):711-9. DOI: 10.1242/jcs.99.4.711. View

14.

Wang M, CUTLER M, Karimpour I, Kleene K . Nucleotide sequence of a mouse testis poly(A) binding protein cDNA. Nucleic Acids Res. 1992; 20(13):3519. PMC: 312519. DOI: 10.1093/nar/20.13.3519. View

15.

Adams T, Hide W, Yager L, Lee B . Isolation of a gene required for programmed initiation of development by Aspergillus nidulans. Mol Cell Biol. 1992; 12(9):3827-33. PMC: 360252. DOI: 10.1128/mcb.12.9.3827-3833.1992. View

16.

Miller K, Wu J, Miller B . StuA is required for cell pattern formation in Aspergillus. Genes Dev. 1992; 6(9):1770-82. DOI: 10.1101/gad.6.9.1770. View

17.

Han S, Navarro J, Greve R, Adams T . Translational repression of brlA expression prevents premature development in Aspergillus. EMBO J. 1993; 12(6):2449-57. PMC: 413480. DOI: 10.1002/j.1460-2075.1993.tb05899.x. View

18.

Sachs A, Wahle E . Poly(A) tail metabolism and function in eucaryotes. J Biol Chem. 1993; 268(31):22955-8. View

19.

Lee B, Adams T . The Aspergillus nidulans fluG gene is required for production of an extracellular developmental signal and is related to prokaryotic glutamine synthetase I. Genes Dev. 1994; 8(6):641-51. DOI: 10.1101/gad.8.6.641. View

20.

Lee B, Adams T . Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development. Mol Microbiol. 1994; 14(2):323-34. DOI: 10.1111/j.1365-2958.1994.tb01293.x. View