Human Osteoclast Formation and Bone Resorption by Monocytes and Synovial Macrophages in Rheumatoid Arthritis

Overview

Journal Ann Rheum Dis

Specialty Rheumatology

Date 1996 Nov 1

PMID 8976638

Citations 43

Authors

Y Fujikawa

A Sabokbar

S Neale

N A Athanasou

Affiliations

Soon will be listed here.

Abstract

Objective: To determine whether synovial macrophages and monocytes isolated from patients with rheumatoid arthritis patients are capable of differentiating into osteoclastic bone resorbing cells; and the cellular and humoral conditions required for this to occur.

Methods: Macrophages isolated from the synovium and monocytes from the peripheral blood of rheumatoid arthritis patients were cultured on bone slices and coverslips, in the presence and absence of UMR 106 rat osteoblast-like cells, 1,25-dihydroxy vitamin D3 (1,25(OH)2D3) and macrophage colony stimulating factor (M-CSF), and assessed for cytochemical and functional evidence of osteoclast differentiation.

Results: Isolated calcitonin receptor (CTR), tartrate resistant acid phosphatase (TRAP), and vitronectin receptor (VNR) negative, CD11b and CD14 positive monocytes and macrophages differentiated into CTR, TRAP, and VNR positive multinucleated cells capable of extensive lacunar bone resorption when co-cultured for 14 d with UMR 106 cells in the presence 1,25(OH)2D3 and M-CSF.

Conclusions: Mononuclear phagocytes (monocytes and macrophages) from rheumatoid arthritis patients are capable of differentiating into multinucleated cells showing all the cytochemical and functional criteria of mature osteoclasts. Synovial macrophage-osteoclast differentiation may represent an important cellular mechanism in the bone destruction associated with rheumatoid arthritis.

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References

Chambers T, Horton M . Failure of cells of the mononuclear phagocyte series to resorb bone. Calcif Tissue Int. 1984; 36(5):556-8. DOI: 10.1007/BF02405365. View

Kotake S, Sato K, Kim K, Takahashi N, Udagawa N, Nakamura I . Interleukin-6 and soluble interleukin-6 receptors in the synovial fluids from rheumatoid arthritis patients are responsible for osteoclast-like cell formation. J Bone Miner Res. 1996; 11(1):88-95. DOI: 10.1002/jbmr.5650110113. View

Horton M, Lewis D, McNulty K, PRINGLE J, Chambers T . Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens. Cancer Res. 1985; 45(11 Pt 2):5663-9. View

Hogg N, Palmer D, Revell P . Mononuclear phagocytes of normal and rheumatoid synovial membrane identified by monoclonal antibodies. Immunology. 1985; 56(4):673-81. PMC: 1453794. View

Baron R, Neff L, Tran Van P, Nefussi J, Vignery A . Kinetic and cytochemical identification of osteoclast precursors and their differentiation into multinucleated osteoclasts. Am J Pathol. 1986; 122(2):363-78. PMC: 1888102. View

Nicholson G, Moseley J, Sexton P, Mendelsohn F, Martin T . Abundant calcitonin receptors in isolated rat osteoclasts. Biochemical and autoradiographic characterization. J Clin Invest. 1986; 78(2):355-60. PMC: 423551. DOI: 10.1172/JCI112584. View

Takahashi N, Yamana H, Yoshiki S, Roodman G, Mundy G, Jones S . Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology. 1988; 122(4):1373-82. DOI: 10.1210/endo-122-4-1373. View

Arnett F, Edworthy S, Bloch D, McShane D, Fries J, Cooper N . The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988; 31(3):315-24. DOI: 10.1002/art.1780310302. View

Henderson B, Revell P, Edwards J . Synovial lining cell hyperplasia in rheumatoid arthritis: dogma and fact. Ann Rheum Dis. 1988; 47(4):348-9. PMC: 1003520. DOI: 10.1136/ard.47.4.348. View

10.

Athanasou N, Quinn J, Heryet A, Puddle B, Woods C, Mcgee J . The immunohistology of synovial lining cells in normal and inflamed synovium. J Pathol. 1988; 155(2):133-42. DOI: 10.1002/path.1711550210. View

11.

Roussel M, Downing J, Rettenmier C, Sherr C . A point mutation in the extracellular domain of the human CSF-1 receptor (c-fms proto-oncogene product) activates its transforming potential. Cell. 1988; 55(6):979-88. DOI: 10.1016/0092-8674(88)90243-7. View

12.

Hale L, Martin M, MCCOLLUM D, Nunley J, Springer T, Singer K . Immunohistologic analysis of the distribution of cell adhesion molecules within the inflammatory synovial microenvironment. Arthritis Rheum. 1989; 32(1):22-30. DOI: 10.1002/anr.1780320105. View

13.

Guerne P, Zuraw B, Vaughan J, Carson D, Lotz M . Synovium as a source of interleukin 6 in vitro. Contribution to local and systemic manifestations of arthritis. J Clin Invest. 1989; 83(2):585-92. PMC: 303718. DOI: 10.1172/JCI113921. View

14.

Van Furth R . Origin and turnover of monocytes and macrophages. Curr Top Pathol. 1989; 79:125-50. View

15.

Xu W, Firestein G, Taetle R, Kaushansky K, Zvaifler N . Cytokines in chronic inflammatory arthritis. II. Granulocyte-macrophage colony-stimulating factor in rheumatoid synovial effusions. J Clin Invest. 1989; 83(3):876-82. PMC: 303761. DOI: 10.1172/JCI113971. View

16.

Athanasou N, Wells C, Quinn J, Ferguson D, Heryet A, Mcgee J . The origin and nature of stromal osteoclast-like multinucleated giant cells in breast carcinoma: implications for tumour osteolysis and macrophage biology. Br J Cancer. 1989; 59(4):491-8. PMC: 2247156. DOI: 10.1038/bjc.1989.102. View

17.

Allen C, Highton J, Palmer D . Increased expression of p150,95 and CR3 leukocyte adhesion molecules by mononuclear phagocytes in rheumatoid synovial membranes. Comparison with osteoarthritic and normal synovial membranes. Arthritis Rheum. 1989; 32(8):947-54. DOI: 10.1002/anr.1780320803. View

18.

Hayes M, Denton J, Freemont A, Mawer E . Synthesis of the active metabolite of vitamin D, 1,25(OH)2D3, by synovial fluid macrophages in arthritic diseases. Ann Rheum Dis. 1989; 48(9):723-9. PMC: 1003863. DOI: 10.1136/ard.48.9.723. View

19.

Sherr C . Colony-stimulating factor-1 receptor. Blood. 1990; 75(1):1-12. View

20.

ZIFF M . Pathways of mononuclear cell infiltration in rheumatoid synovitis. Rheumatol Int. 1989; 9(3-5):97-103. DOI: 10.1007/BF00271865. View