Estimation of DNA Sequence Divergence from Comparison of Restriction Endonuclease Digests

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1977 Jan 1

PMID 896461

Citations 87

Authors

W B Upholt

Affiliations

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Abstract

An estimation of the DNA sequence divergence between defined DNA secquences of individuals or species may be made from comparison by gel electrophoresis of restriction endonuclease digests. This analysis is applicable to purified DNA sequence of moderate complexity (1-100 X 10(6) daltons) which have diverged by base substitution of 0.5 to 25% of nucleotides.

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