Cu2+ Potently Enhances ATP-activated Current in Rat Nodose Ganglion Neurons

Overview

Journal Neurosci Lett

Specialty Neurology

Date 1996 Nov 15

PMID 8961300

Citations 6

Authors

C Li

R W Peoples

F F Weight

Affiliations

Soon will be listed here.

Abstract

Several lines of evidence suggest a physiological role for Cu2+ in regulating nervous system function. In the present study using whole-cell patch-clamp recording, Cu2+ greatly enhanced current activated by 10 microM ATP in the majority of rat nodose ganglion neurons. The enhancement was concentration-dependent between 1 and 50 microM Cu2+, and had an EC50 of 6.1 microM. Cu2+ shifted the ATP concentration-response curve to the left in a parallel manner. However, Cu2+ did not enhance ATP-activated current in the presence of a maximally-effective concentration of Zn2+. The observations suggest that Cu2+ increases the affinity of the receptor for ATP by acting at the Zn2+ modulatory site. In addition, a subset of neurons in the nodose ganglion express ATP-gated receptor-channels that are insensitive to modulation by physiological concentrations of Cu2+, Zn2+ and protons.

Citing Articles

P2X3, but not P2X1, receptors mediate ATP-activated current in neurons innervating tooth-pulp.

Liu Y, Chen X, Tian X, Chen L, Wu Y, Huang D J Huazhong Univ Sci Technolog Med Sci. 2013; 33(3):423-426.

PMID: 23771671 DOI: 10.1007/s11596-013-1135-6.

A P2X receptor from the tardigrade species Hypsibius dujardini with fast kinetics and sensitivity to zinc and copper.

Bavan S, Straub V, Blaxter M, Ennion S BMC Evol Biol. 2009; 9:17.

PMID: 19154569 PMC: 2633282. DOI: 10.1186/1471-2148-9-17.

Dehydroepiandrosterone potentiates native ionotropic ATP receptors containing the P2X2 subunit in rat sensory neurones.

De Roo M, Rodeau J, Schlichter R J Physiol. 2003; 552(Pt 1):59-71.

PMID: 12844512 PMC: 2343311. DOI: 10.1113/jphysiol.2003.046078.

On the contribution of the first transmembrane domain to whole-cell current through an ATP-gated ionotropic P2X receptor.

Haines W, Voigt M, Migita K, Torres G, Egan T J Neurosci. 2001; 21(16):5885-92.

PMID: 11487611 PMC: 6763184.

A domain contributing to the ion channel of ATP-gated P2X2 receptors identified by the substituted cysteine accessibility method.

Egan T, Haines W, Voigt M J Neurosci. 1998; 18(7):2350-9.

PMID: 9502796 PMC: 6793107.