Sex Differences in Benzodiazepine Binding in the Frontal Cortex and Amygdala of the Rat 24 Hours After Restraint Stress

Marked sex differences have been reported in behavioural responses of rats 24 h after exposure to a brief period of restraint (RT) stress. In the present study, differences in benzodiazepine (BZ) binding between male and female rat litter-mates randomly allocated to control or RT groups were investigated 24 h after RT. Scatchard analysis, using [3H] flunitrazepam, was carried out on the the frontal cortex and amygdala. In the frontal cortex, females had a significantly lower affinity and a greater number of BZ receptors than males; males, but not females, showed increased affinity after RT. In the amygdala, there was a tendency towards a greater number of BZ receptors in females, with no effect of RT on receptor number or affinity. These results provide evidence of sex differences in BZ binding both under basal conditions and 24 h after RT, which could contribute to the behavioural sex differences already reported.