Development of a Modified Immunoelectroosmophoresis Method for Uukuniemi and Runde Virus Serology

Overview

Journal Arch Virol

Specialty Microbiology

Date 1977 Jan 1

PMID 889446

Citations 3

Authors

T Traavik

Affiliations

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Abstract

In search for a suitable method for sero-ecological screenings for arboviruses in Norway, efforts were undertaken to make the immunoelectroosmophoresis technique more sensitive than here to fare in detection of antibodies. The aim was to make it comparable to haemagglutination inhibition test in sensitivity, retaining the advantages in specificity, simplicity and capacity. This has been achieved by: 1. Using concentrated virus as antigen. 2. Performing electrophoresis in a gel consisting of an agar-agarose mixture in optimal concentrations. 3. "Sandwiching" the first specific electrophoretic run with an anti-species antiserum to the tested sample.

Citing Articles

Evidence for flavivirus(es) outside of the distribution area for Ixodes ricinus in Norway.

Traavik T, Wiger R, Mehl R J Hyg (Lond). 1984; 93(1):133-8.

PMID: 6086747 PMC: 2129272. DOI: 10.1017/s0022172400061015.

Mosquito-borne arboviruses in Norway: further isolations and detection of antibodies to California encephalitis viruses in human, sheep and wildlife sera.

Traavik T, Mehl R, Wiger R J Hyg (Lond). 1985; 94(1):111-22.

PMID: 2857742 PMC: 2129400. DOI: 10.1017/s0022172400061180.

"Runde" viurs, a coronavirus-like agent associated with seabirds and ticks.

Traavik T, Mehl R, Kjeldsberg E Arch Virol. 1977; 55(1-2):25-38.

PMID: 72555 PMC: 7087166. DOI: 10.1007/BF01314476.

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