Cell Cycle Stage-specific Phosphorylation of the Epstein-Barr Virus Immortalization Protein EBNA-LP

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1996 Nov 1

PMID 8892911

Citations 27

Authors

M K Kitay

D T Rowe

Affiliations

Soon will be listed here.

Abstract

EBNA-LP is a viral nuclear oncoprotein implicated in the immortalization of B lymphocytes by Epstein-Barr virus. An analysis of EBNA-LP migration on polyacrylamide gels was performed with protein derived from the X50-7 lymphoblastoid cell line blocked by hydroxyurea or aphidicolin at the G1/S phase of the cell cycle or by nocodazole at the G2/M phase. More slowly migrating species of EBNA-LP were detected in G2/M phase-arrested cell extracts. Release from nocodazole G2/M block or treatment with phosphatase caused the more slowly migrating species of EBNA-LP to disappear. Analyses of 32PO(4)(3-)-labeled EBNA-LP protein immunoprecipitated from the drug-synchronized cells showed that phosphorylated EBNA-LP was present throughout the cell cycle but that phosphorylation increased in G2 and was maximal at G2/M. Phosphoamino acid analysis revealed that all phosphorylation was on serine residues only. The ability of EBNA-LP to be phosphorylated by p34(cdc2) kinase and casein kinase II exclusively on serines implicates these enzymes as being potentially involved in EBNA-LP phosphorylation.

Citing Articles

Epstein-Barr virus protein EBNA-LP engages YY1 through leucine-rich motifs to promote naïve B cell transformation.

Cable J, Reinoso-Vizcaino N, White R, Luftig M PLoS Pathog. 2024; 20(7):e1011950.

PMID: 39083560 PMC: 11318927. DOI: 10.1371/journal.ppat.1011950.

Epstein-Barr virus protein EBNA-LP engages YY1 through leucine-rich motifs to promote naïve B cell transformation.

Cable J, Reinoso-Vizcaino N, White R, Luftig M bioRxiv. 2024; .

PMID: 38260266 PMC: 10802455. DOI: 10.1101/2024.01.07.574580.

Functional diversity: update of the posttranslational modification of Epstein-Barr virus coding proteins.

Sun Y, Liu W, Luo B Cell Mol Life Sci. 2022; 79(12):590.

PMID: 36376593 PMC: 11802978. DOI: 10.1007/s00018-022-04561-2.

Molecular mechanisms of EBV-driven cell cycle progression and oncogenesis.

Yin H, Qu J, Peng Q, Gan R Med Microbiol Immunol. 2018; 208(5):573-583.

PMID: 30386928 PMC: 6746687. DOI: 10.1007/s00430-018-0570-1.

Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle.

Chiu H, Huang W, Liao T, Wu H, Munir M, Shih W PLoS One. 2016; 11(9):e0162356.

PMID: 27603133 PMC: 5014334. DOI: 10.1371/journal.pone.0162356.

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