S-formylglutathione Hydrolase of Paracoccus Denitrificans is Homologous to Human Esterase D: a Universal Pathway for Formaldehyde Detoxification?

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1996 Nov 1

PMID 8892832

Citations 50

Authors

N Harms

J Ras

W N Reijnders

R J Van Spanning

A H Stouthamer

Affiliations

Soon will be listed here.

Abstract

Downstream of flhA, the Paracoccus denitrificans gene encoding glutathione-dependent formaldehyde dehydrogenase, an open reading frame was identified and called fghA. The gene product of fghA showed appreciable similarity with human esterase D and with the deduced amino acid sequences of open reading frames found in Escherichia coli, Haemophilus influenzae, and Saccharomyces cerevisiae. Mutating fghA strongly reduced S-formylglutathione hydrolase activity. The mutant was unable to grow on methanol and methylamine, indicating that the enzyme is essential for methylotrophic growth. S-Formylglutathione hydrolase appears to be part of a formaldehyde detoxification pathway that is universal in nature.

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References

Maurizi M, Clark W, Kim S, Gottesman S . Clp P represents a unique family of serine proteases. J Biol Chem. 1990; 265(21):12546-52. View

SANGER F, Coulson A, Barrell B, Smith A, Roe B . Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980; 143(2):161-78. DOI: 10.1016/0022-2836(80)90196-5. View

Harms N, Van Spanning R . C1 metabolism in Paracoccus denitrificans: genetics of Paracoccus denitrificans. J Bioenerg Biomembr. 1991; 23(2):187-210. DOI: 10.1007/BF00762217. View

Nakazono M, Hirai A . Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice. Mol Gen Genet. 1993; 236(2-3):341-6. DOI: 10.1007/BF00277131. View

Gish W, States D . Identification of protein coding regions by database similarity search. Nat Genet. 1993; 3(3):266-72. DOI: 10.1038/ng0393-266. View

Giffard P, Rathsam C, Kwan E, Kwan D, Bunny K, Koo S . The ftf gene encoding the cell-bound fructosyltransferase of Streptococcus salivarius ATCC 25975 is preceded by an insertion sequence and followed by FUR1 and clpP homologues. J Gen Microbiol. 1993; 139(5):913-20. DOI: 10.1099/00221287-139-5-913. View

Ito K, Takahashi M, Yoshimoto T, Tsuru D . Cloning and high-level expression of the glutathione-independent formaldehyde dehydrogenase gene from Pseudomonas putida. J Bacteriol. 1994; 176(9):2483-91. PMC: 205383. DOI: 10.1128/jb.176.9.2483-2491.1994. View

Speer B, Chistoserdova L, Lidstrom M . Sequence of the gene for a NAD(P)-dependent formaldehyde dehydrogenase (class III alcohol dehydrogenase) from a marine methanotroph Methylobacter marinus A45. FEMS Microbiol Lett. 1994; 121(3):349-55. DOI: 10.1111/j.1574-6968.1994.tb07125.x. View

Ras J, van Ophem P, Reijnders W, Van Spanning R, Duine J, Stouthamer A . Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth. J Bacteriol. 1995; 177(1):247-51. PMC: 176581. DOI: 10.1128/jb.177.1.247-251.1995. View

10.

Yanase H, Noda H, Aoki K, Kita K, Kato N . Cloning, sequence analysis, and expression of the gene encoding formaldehyde dismutase from Pseudomonas putida F61. Biosci Biotechnol Biochem. 1995; 59(2):197-202. DOI: 10.1271/bbb.59.197. View

11.

Sakai Y, Murdanoto A, Sembiring L, Tani Y, Kato N . A novel formaldehyde oxidation pathway in methylotrophic yeasts: methylformate as a possible intermediate. FEMS Microbiol Lett. 1995; 127(3):229-34. DOI: 10.1111/j.1574-6968.1995.tb07478.x. View

12.

Chang J, Morris J . Studies on the utilization of nitrate by Micrococcus denitrificans. J Gen Microbiol. 1962; 29:301-10. DOI: 10.1099/00221287-29-2-301. View

13.

Van Spanning R, Wansell C, Harms N, Oltmann L, Stouthamer A . Mutagenesis of the gene encoding cytochrome c550 of Paracoccus denitrificans and analysis of the resultant physiological effects. J Bacteriol. 1990; 172(2):986-96. PMC: 208527. DOI: 10.1128/jb.172.2.986-996.1990. View

14.

Uotila L, Koivusalo M . Purification and properties of S-formylglutathione hydrolase from human liver. J Biol Chem. 1974; 249(23):7664-72. View

15.

Markwell M, Haas S, Bieber L, Tolbert N . A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978; 87(1):206-10. DOI: 10.1016/0003-2697(78)90586-9. View

16.

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

17.

Lee E, Lee W . Molecular cloning of the human esterase D gene, a genetic marker of retinoblastoma. Proc Natl Acad Sci U S A. 1986; 83(17):6337-41. PMC: 386498. DOI: 10.1073/pnas.83.17.6337. View

18.

Lee W, Wheatley W, Benedict W, Huang C, Lee E . Purification, biochemical characterization, and biological function of human esterase D. Proc Natl Acad Sci U S A. 1986; 83(18):6790-4. PMC: 386595. DOI: 10.1073/pnas.83.18.6790. View

19.

Eiberg H, Mohr J . Identity of the polymorphisms for esterase D and S-formylglutathione hydrolase in red blood cells. Hum Genet. 1986; 74(2):174-5. DOI: 10.1007/BF00282085. View

20.

de Vries G, Harms N, Maurer K, Papendrecht A, Stouthamer A . Physiological regulation of Paracoccus denitrificans methanol dehydrogenase synthesis and activity. J Bacteriol. 1988; 170(8):3731-7. PMC: 211352. DOI: 10.1128/jb.170.8.3731-3737.1988. View