Assay of Free-radical Toxicity and Antioxidant Effect on the Hep 3B Cell Line: a Test Survey Using Lindane

Overview

Journal Cell Biol Toxicol

Publisher Springer

Specialties Cell Biology
Toxicology

Date 1996 Feb 1

PMID 8882386

Citations 1

Authors

B Descampiaux

J M Leroux

C Peucelle

F Erb

Affiliations

Soon will be listed here.

Abstract

The content of reduced glutathione and of glutathione disulfide as well as the activities of glutathione reductase, glutathione peroxidase, glutathione S-transferases, catalase and superoxide dismutases were determined in human hepatoma Hep 3B cells in relation to free-radical toxicity in order to appreciate the defense capacities of these cells compared to data on normal hepatocytes. When Hep 3B cells were exposed to lindane, a known inducer of free-radical production, superoxide dismutase activity appeared as the best-adapted cellular parameter for early detection of the resulting free-radical toxicity.

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PMID: 10428683 PMC: 3547643.

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