Chemiluminescent and Flow Cytometric Analysis of Gamma Interferon Preincubation on Neonatal and Adult Rat Polymorphonuclear Leukocytes

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1996 Sep 1

PMID 8877130

Authors

R R Wittler

M M Lieberman

D D Paine

S L Muehlbauer

J E Lima

D M Sachanandani

C A Pinney

Affiliations

Soon will be listed here.

Abstract

Gamma interferon (IFN-gamma) has multiple immunomodulating effects and has been postulated as a possible immunopotentiating agent for the prevention or treatment of neonatal infections. This report describes the effect of rat recombinant IFN-gamma on the oxidative burst activity and CD11b expression of neonatal and adult rat polymorphonuclear leukocytes (PMNL). Oxidative burst activity was assessed by chemiluminescence and dihydrorhodamine flow cytometry. Neonatal PMNL exhibited significantly less oxidative burst activity than did adult PMNL. IFN-gamma mildly enhanced the chemiluminescence response of PMNL from both the rat pups and adults, but this effect was not statistically significant when analyzed by a multivariate model of repeated-measures analysis of variance for both chemiluminescence and dihydrorhodamine flow cytometry. CD11b expression was also not significantly enhanced by IFN-gamma.

References

Pahwa S, Pahwa R, Grimes E, SMITHWICK E . Cellular and humoral components of monocyte and neutrophil chemotaxis in cord blood. Pediatr Res. 1977; 11(5):677-80. DOI: 10.1203/00006450-197705000-00010. View

Quie P . Antimicrobial defenses in the neonate. Semin Perinatol. 1990; 14(4 Suppl 1):2-9. View

Welch W . Correlation between measurements of the luminol-dependent chemiluminescence response and bacterial susceptibility to phagocytosis. Infect Immun. 1980; 30(2):370-4. PMC: 551320. DOI: 10.1128/iai.30.2.370-374.1980. View

Sacchi F, Hill H . Defective membrane potential changes in neutrophils from human neonates. J Exp Med. 1984; 160(4):1247-52. PMC: 2187475. DOI: 10.1084/jem.160.4.1247. View

Matricardi P, Capobianchi M, Paganelli R, Facchini J, Sirianni M, Seminara R . Interferon production in primary immunodeficiencies. J Clin Immunol. 1984; 4(5):388-94. DOI: 10.1007/BF00917142. View

Wilson C . Immunologic basis for increased susceptibility of the neonate to infection. J Pediatr. 1986; 108(1):1-12. DOI: 10.1016/s0022-3476(86)80761-2. View

Anderson D, Freeman K, Heerdt B, Hughes B, Jack R, Smith C . Abnormal stimulated adherence of neonatal granulocytes: impaired induction of surface Mac-1 by chemotactic factors or secretagogues. Blood. 1987; 70(3):740-50. View

Peden D, Vandyke K, Ardekani A, Mullett M, Myerberg D, VanDyke C . Diminished chemiluminescent responses of polymorphonuclear leukocytes in severely and moderately preterm neonates. J Pediatr. 1987; 111(6 Pt 1):904-6. DOI: 10.1016/s0022-3476(87)80216-0. View

Bortolussi R, Issekutz T, Burbridge S, Schellekens H . Neonatal host defense mechanisms against Listeria monocytogenes infection: the role of lipopolysaccharides and interferons. Pediatr Res. 1989; 25(3):311-5. DOI: 10.1203/00006450-198903000-00021. View

10.

Weisman L, Lorenzetti P . High intravenous doses of human immune globulin suppress neonatal group B streptococcal immunity in rats. J Pediatr. 1989; 115(3):445-50. DOI: 10.1016/s0022-3476(89)80853-4. View

11.

Buckle A, Hogg N . The effect of IFN-gamma and colony-stimulating factors on the expression of neutrophil cell membrane receptors. J Immunol. 1989; 143(7):2295-301. View

12.

Dijkmans R, Billiau A . Interferon gamma: a master key in the immune system. Curr Opin Immunol. 1988; 1(2):269-74. DOI: 10.1016/0952-7915(88)90013-1. View

13.

Del Prete G, Tiri A, Maggi E, De Carli M, Macchia D, Parronchi P . Defective in vitro production of gamma-interferon and tumor necrosis factor-alpha by circulating T cells from patients with the hyper-immunoglobulin E syndrome. J Clin Invest. 1989; 84(6):1830-5. PMC: 304061. DOI: 10.1172/JCI114368. View

14.

Driscoll M, Thomas V, Ramamurthy R, Casto D . Longitudinal evaluation of polymorphonuclear leukocyte chemiluminescence in premature infants. J Pediatr. 1990; 116(3):429-34. DOI: 10.1016/s0022-3476(05)82838-0. View

15.

Buckle A, Hogg N . Human memory T cells express intercellular adhesion molecule-1 which can be increased by interleukin 2 and interferon-gamma. Eur J Immunol. 1990; 20(2):337-41. DOI: 10.1002/eji.1830200216. View

16.

Mease A . Tissue neutropenia: the newborn neutrophil in perspective. J Perinatol. 1990; 10(1):55-9. View

17.

Cairo M, Mauss D, Kommareddy S, Norris K, van de Ven C, Modanlou H . Prophylactic or simultaneous administration of recombinant human granulocyte colony stimulating factor in the treatment of group B streptococcal sepsis in neonatal rats. Pediatr Res. 1990; 27(6):612-6. DOI: 10.1203/00006450-199006000-00016. View

18.

Wilson C, Lewis D . Basis and implications of selectively diminished cytokine production in neonatal susceptibility to infection. Rev Infect Dis. 1990; 12 Suppl 4:S410-20. DOI: 10.1093/clinids/12.supplement_4.s410. View

19.

Fischer G, Hemming V, Gloser H, Bachmayer H, von Pilar C, Wilson S . Polyvalent group B streptococcal immune globulin for intravenous administration: overview. Rev Infect Dis. 1990; 12 Suppl 4:S483-9; discussion S489-91. DOI: 10.1093/clinids/12.supplement_4.s483. View

20.

Lindena J, Burkhardt H . Chemiluminescence properties of human, canine and rat polymorphonuclear cells. J Biolumin Chemilumin. 1990; 5(3):165-70. DOI: 10.1002/bio.1170050304. View