Transposition of DNase Hypersensitive Chromatin to the Nuclear Periphery Coincides Temporally with Nerve Growth Factor-induced Up-regulation of Gene Expression in PC12 Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1996 Oct 15

PMID 8876190

Citations 2

Authors

P C Park

U De Boni

Affiliations

Soon will be listed here.

Abstract

To test the hypothesis that the nonrandom organization of the contents of interphase nuclei represents a compartmentalization of function, we examined the relative, spatial relationship of small nuclear ribonucleoproteins (snRNPs) and of DNase I hypersensitive chromatin (DHC) in rat pheochromocytoma cells. In controls, DHC and snRNPs colocalized as pan-nuclear speckles. During nerve growth factor-induced differentiation, both snRNPs and DHC migrated to the nuclear periphery with the migration of DHC preceding that of snRNPs, resulting in their transient separation. The formation of DHC shells temporally coincided with an up-regulation of neurofilament light chain mRNA. This indicates that the expression of this sequence may be associated with its spatial transposition to the nuclear periphery.

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