Typing of Helicobacter Pylori with Monoclonal Antibodies Against Lewis Antigens in Lipopolysaccharide

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1996 Sep 1

PMID 8862584

Citations 51

Authors

B J Appelmelk

T Verboom

R Negrini

J L Penner

G O ASPINALL

A P Moran

S F Fei

B S Shi

W Rudnica

A Savio

J de Graaff

Affiliations

Soon will be listed here.

Abstract

Recently, it has been shown that the lipopolysaccharide (LPS) O antigen of Helicobacter pylori contains Lewis x (Lex), Lewis y (Ley), or both Lex and Ley antigens. We applied a serotyping method for H. pylori by an enzyme-linked immunosorbent assay with monoclonal antibodies (MAbs) specific for these antigens and the related fucosylated H type 1 (H1) antigen. The selected MAbs recognized the Lex and/or Ley structures in the LPS of H. pylori. The agreement between the results of biochemical compositional analysis and the serological data validated our serotyping system. A total of 152 strains from different geographic origins (The Netherlands, Canada, Poland, Italy, and People's Republic of China) were examined for typeability based on the presence of Lewis antigens. One hundred twenty-nine (84.9%) strains were typeable, and 12 different serotyping patterns were observed; 80.9% of the strains contained Lex and/or Le(y) antigens, and 18.4% reacted with the MAb against the related H1 antigen either alone or in combination with the Lex and/or Ley antigen. Our results show that the Lex and Ley antigens are frequently encountered in the LPS of H. pylori strains from various geographic origins. This typing method is an easy-to-perform technique, which can be used for strain differentiation in epidemiological studies of H. pylori infections.

Citing Articles

glycan biosynthesis modulates host immune cell recognition and response.

Barrett K, Kassama F, Surks W, Mulholland A, Moulton K, Dube D Front Cell Infect Microbiol. 2024; 14:1377077.

PMID: 38572314 PMC: 10987845. DOI: 10.3389/fcimb.2024.1377077.

Lipopolysaccharide O-antigen profiles of Helicobacter pylori strains from Southwest China.

Tang X, Wang P, Shen Y, Song X, Benghezal M, Marshall B BMC Microbiol. 2023; 23(1):360.

PMID: 37993791 PMC: 10664510. DOI: 10.1186/s12866-023-03116-0.

Anti-Glycan Autoantibodies Induced by as a Potential Risk Factor for Myocardial Infarction.

Negrini R, Villanacci V, Poiesi C, Savio A Front Immunol. 2020; 11:597.

PMID: 32322255 PMC: 7158853. DOI: 10.3389/fimmu.2020.00597.

Upregulation of MUC5AC production and deposition of LEWIS determinants by HELICOBACTER PYLORI facilitate gastric tissue colonization and the maintenance of infection.

Gonciarz W, Walencka M, Moran A, Hinc K, Obuchowski M, Chmiela M J Biomed Sci. 2019; 26(1):23.

PMID: 30841890 PMC: 6402143. DOI: 10.1186/s12929-019-0515-z.

Diagnosis of Using Invasive and Noninvasive Approaches.

Talebi Bezmin Abadi A J Pathog. 2018; 2018:9064952.

PMID: 29951318 PMC: 5987299. DOI: 10.1155/2018/9064952.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Tsai C, Frasch C . A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982; 119(1):115-9. DOI: 10.1016/0003-2697(82)90673-x. View

Hitchcock P, Brown T . Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983; 154(1):269-77. PMC: 217456. DOI: 10.1128/jb.154.1.269-277.1983. View

Megraud F, Bonnet F, Garnier M, Lamouliatte H . Characterization of "Campylobacter pyloridis" by culture, enzymatic profile, and protein content. J Clin Microbiol. 1985; 22(6):1007-10. PMC: 271867. DOI: 10.1128/jcm.22.6.1007-1010.1985. View

Langenberg W, Rauws E, Widjojokusumo A, Tytgat G, Zanen H . Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J Clin Microbiol. 1986; 24(3):414-7. PMC: 268925. DOI: 10.1128/jcm.24.3.414-417.1986. View

Perez-Perez G, Blaser M . Conservation and diversity of Campylobacter pyloridis major antigens. Infect Immun. 1987; 55(5):1256-63. PMC: 260499. DOI: 10.1128/iai.55.5.1256-1263.1987. View

McNulty C, Dent J . Rapid identification of Campylobacter pylori (C. pyloridis) by preformed enzymes. J Clin Microbiol. 1987; 25(9):1683-6. PMC: 269307. DOI: 10.1128/jcm.25.9.1683-1686.1987. View

Bara J, Daher N, Mollicone R, Oriol R . Immunohistological patterns of 20 monoclonal antibodies against non-A non-B glycoconjugates in normal human pyloric and duodenal mucosae. Rev Fr Transfus Immunohematol. 1987; 30(5):685-92. DOI: 10.1016/s0338-4535(87)80136-8. View

Danielsson D, Blomberg B, Jarnerot G, Kosunen T . Heterogeneity of Campylobacter pylori as demonstrated by co-agglutination testing with rabbit antibodies. Scand J Gastroenterol Suppl. 1988; 142:58-63. View

10.

Burnie J, Lee W, Dent J, McNulty C . Immunoblot fingerprinting of Campylobacter pylori. J Med Microbiol. 1988; 27(2):153-9. DOI: 10.1099/00222615-27-2-153. View

11.

Bara J, Gautier R, Le Pendu J, Oriol R . Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988; 254(1):185-93. PMC: 1135055. DOI: 10.1042/bj2540185. View

12.

Negrini R, Lisato L, Cavazzini L, Maini P, Gullini S, Basso O . Monoclonal antibodies for specific immunoperoxidase detection of Campylobacter pylori. Gastroenterology. 1989; 96(2 Pt 1):414-20. DOI: 10.1016/0016-5085(89)91565-5. View

13.

Kung J, Ho B, Chan S . Biotyping of Campylobacter pylori. J Med Microbiol. 1989; 29(3):203-6. DOI: 10.1099/00222615-29-3-203. View

14.

Buck G . Campylobacter pylori and gastroduodenal disease. Clin Microbiol Rev. 1990; 3(1):1-12. PMC: 358137. DOI: 10.1128/CMR.3.1.1. View

15.

Costas M, Owen R, Bickley J, Morgan D . Molecular techniques for studying the epidemiology of infection by Helicobacter pylori. Scand J Gastroenterol Suppl. 1991; 181:20-32. DOI: 10.3109/00365529109093204. View

16.

Parsonnet J, Friedman G, Vandersteen D, Chang Y, Vogelman J, ORENTREICH N . Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991; 325(16):1127-31. DOI: 10.1056/NEJM199110173251603. View

17.

Morgan D, Costas M, Owen R, Williams E . Characterization of strains of Helicobacter pylori: one-dimensional SDS-PAGE as a molecular epidemiologic tool. Rev Infect Dis. 1991; 13 Suppl 8:S709-13. DOI: 10.1093/clinids/13.supplement_8.s709. View

18.

Wotherspoon A, Ortiz-Hidalgo C, Falzon M, Isaacson P . Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. Lancet. 1991; 338(8776):1175-6. DOI: 10.1016/0140-6736(91)92035-z. View

19.

Moran A, Helander I, Kosunen T . Compositional analysis of Helicobacter pylori rough-form lipopolysaccharides. J Bacteriol. 1992; 174(4):1370-7. PMC: 206434. DOI: 10.1128/jb.174.4.1370-1377.1992. View

20.

Tee W, Lambert J, Smallwood R, Schembri M, Ross B, Dwyer B . Ribotyping of Helicobacter pylori from clinical specimens. J Clin Microbiol. 1992; 30(6):1562-7. PMC: 265329. DOI: 10.1128/jcm.30.6.1562-1567.1992. View