Multiple Components of Arginine and Phenylalanine Transport Induced in Neutral and Basic Amino Acid Transporter-cRNA-injected Xenopus Oocytes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1996 Sep 15

PMID 8836138

Citations 6

Authors

G J Peter

I G DAVIDSON

A Ahmed

L McIlroy

A R FORRESTER

P M Taylor

Affiliations

Soon will be listed here.

Abstract

The induced uptakes of L-[3H]phenylalanine and L-[3H]arginine in oocytes injected with clonal NBAT (neutral and basic amino acid transporter) cRNA show differential inactivation by pretreatment with N-ethylmaleimide (NEM), revealing at least two distinct transport processes. NEM-resistant arginine transport is inhibited by leucine and phenylalanine but not by alanine or valine; mutual competitive inhibition of NEM-resistant uptake of arginine and phenylalanine indicates that the two amino acids share a single transporter. NEM-sensitive arginine transport is inhibited by leucine, phenylalanine, alanine and valine. At least two NEM-sensitive transporters may be expressed because we have been unable to confirm mutual competitive inhibition between arginine and phenylalanine transport. The NEM-resistant transport mechanism appears to involve distinct but overlapping binding sites for cationic and zwitterionic substrates. NBAT is known to form oligomeric protein complexes in cell membranes, and its functional roles when expressed in Xenopus oocytes may include interaction with oocyte proteins, leading to increased native amino acid transport activities; these resemble NBAT-expressed activities in terms of NEM-sensitivity and apparent substrate range (including an unusual inhibition by beta-phenylalanine.

Citing Articles

Evidence for allosteric regulation of pH-sensitive System A (SNAT2) and System N (SNAT5) amino acid transporter activity involving a conserved histidine residue.

Baird F, Pinilla-Tenas J, Ogilvie W, Ganapathy V, Hundal H, Taylor P Biochem J. 2006; 397(2):369-75.

PMID: 16629640 PMC: 1513278. DOI: 10.1042/BJ20060026.

Bidirectional substrate fluxes through the system N (SNAT5) glutamine transporter may determine net glutamine flux in rat liver.

Baird F, Beattie K, Hyde A, Ganapathy V, Rennie M, Taylor P J Physiol. 2004; 559(Pt 2):367-81.

PMID: 15218073 PMC: 1665133. DOI: 10.1113/jphysiol.2003.060293.

Cysteine residues in the C-terminus of the neutral- and basic-amino-acid transporter heavy-chain subunit contribute to functional properties of the system b(0,+)-type amino acid transporter.

Peter G, Panova T, Christie G, Taylor P Biochem J. 2000; 351 Pt 3:677-82.

PMID: 11042122 PMC: 1221407.

Interactions between the thiol-group reagent N-ethylmaleimide and neutral and basic amino acid transporter-related amino acid transport.

Peter G, Davies A, Watt P, Birrell J, Taylor P Biochem J. 1999; 343 Pt 1:169-76.

PMID: 10493926 PMC: 1220538.

Discrimination of two amino acid transport activities in 4F2 heavy chain- expressing Xenopus laevis oocytes.

Broer A, Hamprecht B, Broer S Biochem J. 1998; 333 ( Pt 3):549-54.

PMID: 9677312 PMC: 1219616. DOI: 10.1042/bj3330549.

References

Coady M, Jalal F, Chen X, Lemay G, Berteloot A, Lapointe J . Electrogenic amino acid exchange via the rBAT transporter. FEBS Lett. 1994; 356(2-3):174-8. DOI: 10.1016/0014-5793(94)01262-8. View

Calonge M, Gasparini P, Chillaron J, Chillon M, Gallucci M, Rousaud F . Cystinuria caused by mutations in rBAT, a gene involved in the transport of cystine. Nat Genet. 1994; 6(4):420-5. DOI: 10.1038/ng0494-420. View

Ahmed A, Peter G, Taylor P, Harper A, Rennie M . Sodium-independent currents of opposite polarity evoked by neutral and cationic amino acids in neutral and basic amino acid transporter cRNA-injected oocytes. J Biol Chem. 1995; 270(15):8482-6. DOI: 10.1074/jbc.270.15.8482. View

Fei Y, Prasad P, Leibach F, Ganapathy V . The amino acid transport system y+L induced in Xenopus laevis oocytes by human choriocarcinoma cell (JAR) mRNA is functionally related to the heavy chain of the 4F2 cell surface antigen. Biochemistry. 1995; 34(27):8744-51. DOI: 10.1021/bi00027a025. View

Wang Y, Tate S . Oligomeric structure of a renal cystine transporter: implications in cystinuria. FEBS Lett. 1995; 368(2):389-92. DOI: 10.1016/0014-5793(95)00685-3. View

Waldegger S, Schmidt F, Herzer T, Gulbins E, Schuster A, Biber J . Heavy metal mediated inhibition of rBAT-induced amino acid transport. Kidney Int. 1995; 47(6):1677-81. DOI: 10.1038/ki.1995.232. View

Miyamoto K, Katai K, Tatsumi S, Sone K, Segawa H, Yamamoto H . Mutations of the basic amino acid transporter gene associated with cystinuria. Biochem J. 1995; 310 ( Pt 3):951-5. PMC: 1135988. DOI: 10.1042/bj3100951. View

Fincham D, Mason D, Paterson J, Young J . Heterogeneity of amino acid transport in horse erythrocytes: a detailed kinetic analysis of inherited transport variation. J Physiol. 1987; 389:385-409. PMC: 1192086. DOI: 10.1113/jphysiol.1987.sp016662. View

Fincham D, Mason D, Young J . Characterization of a novel Na+-independent amino acid transporter in horse erythrocytes. Biochem J. 1985; 227(1):13-20. PMC: 1144803. DOI: 10.1042/bj2270013. View

10.

Broer S, Broer A, Hamprecht B . The 4F2hc surface antigen is necessary for expression of system L-like neutral amino acid-transport activity in C6-BU-1 rat glioma cells: evidence from expression studies in Xenopus laevis oocytes. Biochem J. 1995; 312 ( Pt 3):863-70. PMC: 1136194. DOI: 10.1042/bj3120863. View

11.

Young J, Mason D, Fincham D . Topographical similarities between harmaline inhibition sites on Na+-dependent amino acid transport system ASC in human erythrocytes and Na+-independent system asc in horse erythrocytes. J Biol Chem. 1988; 263(1):140-3. View

12.

Van Winkle L, Campione A, Gorman J . Na+-independent transport of basic and zwitterionic amino acids in mouse blastocysts by a shared system and by processes which distinguish between these substrates. J Biol Chem. 1988; 263(7):3150-63. View

13.

Campa M, Kilberg M . Characterization of neutral and cationic amino acid transport in Xenopus oocytes. J Cell Physiol. 1989; 141(3):645-52. DOI: 10.1002/jcp.1041410324. View

14.

Geering K, Theulaz I, Verrey F, Hauptle M, Rossier B . A role for the beta-subunit in the expression of functional Na+-K+-ATPase in Xenopus oocytes. Am J Physiol. 1989; 257(5 Pt 1):C851-8. DOI: 10.1152/ajpcell.1989.257.5.C851. View

15.

Van Winkle L, Campione A, Gorman J . Inhibition of transport system b0,+ in blastocysts by inorganic and organic cations yields insight into the structure of its amino acid receptor site. Biochim Biophys Acta. 1990; 1025(2):215-24. DOI: 10.1016/0005-2736(90)90100-3. View

16.

Van Winkle L, Mann D, Campione A, Farrington B . Transport of benzenoid amino acids by system T and four broad scope systems in preimplantation mouse conceptuses. Biochim Biophys Acta. 1990; 1027(3):268-77. DOI: 10.1016/0005-2736(90)90318-i. View

17.

Tate S, Yan N, UDENFRIEND S . Expression cloning of a Na(+)-independent neutral amino acid transporter from rat kidney. Proc Natl Acad Sci U S A. 1992; 89(1):1-5. PMC: 48162. DOI: 10.1073/pnas.89.1.1. View

18.

Wells R, Hediger M . Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases. Proc Natl Acad Sci U S A. 1992; 89(12):5596-600. PMC: 49339. DOI: 10.1073/pnas.89.12.5596. View

19.

Bertran J, Werner A, Moore M, Stange G, Markovich D, Biber J . Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids. Proc Natl Acad Sci U S A. 1992; 89(12):5601-5. PMC: 49340. DOI: 10.1073/pnas.89.12.5601. View

20.

Wells R, Lee W, Kanai Y, Leiden J, Hediger M . The 4F2 antigen heavy chain induces uptake of neutral and dibasic amino acids in Xenopus oocytes. J Biol Chem. 1992; 267(22):15285-8. View