Effect of TraY Amber Mutations on F-plasmid TraY Promoter Activity in Vivo

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1996 Oct 1

PMID 8824627

Citations 7

Authors

P M Silverman

A Sholl

Affiliations

Soon will be listed here.

Abstract

We have examined the effect of the F plasmid TraY protein on tra gene expression in vivo. Expression was assayed as alkaline phosphatase activity in cells containing a traY phi(traA'-'phoA)hyb operon under traY promoter control. Amber mutations in traY significantly reduced alkaline phosphatase activity. Since nonsense polarity effects were minimal, if they occurred at all, these data provide the first direct evidence that TraY regulates tra gene expression.

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