The UvrB Gene of Pseudomonas Aeruginosa is Not DNA Damage Inducible

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1996 Sep 1

PMID 8808952

Citations 6

Authors

E Rivera

L Vila

J Barbe

Affiliations

Soon will be listed here.

Abstract

The uvrB gene of Pseudomonas aeruginosa has been isolated from a genomic library by complementation of an Escherichia coli uvrB mutant. The complete nucleotide sequence of P. aeruginosa uvrB consists of 2,013 bp, encoding a polypeptide of 670 amino acids. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, is not present in the upstream region of the uvrB gene isolated. By transcriptional fusions with a reporter gene, it has been demonstrated that, contrary to what happens with the homologous gene of E. coli, the P. aeruginosa uvrB gene is not DNA damage inducible. Nevertheless, the UvrB protein must be functional in P. aeruginosa cells because a uvrB-defective mutant is extremely sensitive to UV radiation.

Citing Articles

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DNA repair of 8-oxo-7,8-dihydroguanine lesions in Porphyromonas gingivalis.

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Survival of Shewanella oneidensis MR-1 after UV radiation exposure.

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Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene.

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Characterization of the serogroup O11 O-antigen locus of Pseudomonas aeruginosa PA103.

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