Purification and Characterization of Two Transcribed Repetitive DNA Fractions from the Pigeon Genome

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1977 Jun 23

PMID 880844

Authors

K G Gasaryan

E D Kuznetsova

V Z Tarantul

S A Sivak

Affiliations

Soon will be listed here.

Abstract

Two fractions of the repeats belonging to intermediate frequency repetitive DNA were isolated from the total pigeon nuclear DNA fragmented to about 450 nucleotides. One fraction was designated as "rare repeats" (repetition frequency about 35 per haploid genome) and another termed as "moderate repeats" (repetition frequency about 2500 per haploid genome). The rare repeats, which constitute about 7% of the total DNA, include at least 75% of the repetitive DNA sequences transcribed into the high molecular fraction (greater than 45S) of HnRNA in erythroid cells. These repeats have properties compatible with the characteristics of the class of low frequency interspersed DNA found in genomes of many other Metazoan species. The moderate repeats contribute only about 10-20% of the total repetitive DNA copies present in greater than 45S HnRNA and differ from the rare repeats in some other properties.--The possible role of the rare repeats in the genome is discussed.

References

Bishop J, Freeman K . DNA sequences neighboring the duck hemoglobin genes. Cold Spring Harb Symp Quant Biol. 1974; 38:707-16. DOI: 10.1101/sqb.1974.038.01.076. View

Davidson E, HOUGH B, Klein W, Britten R . Structural genes adjacent to interspersed repetitive DNA sequences. Cell. 1975; 4(3):217-38. DOI: 10.1016/0092-8674(75)90170-1. View

. Simplification of the empirical relationship between melting temperature of DNA, its GC content and concentration of sodium ions in solution. Biopolymers. 1971; 10(12):2623-4. DOI: 10.1002/bip.360101223. View

Klein W, Murphy W, Attardi G, Britten R, Davidson E . Distribution of repetitive and nonrepetivite sequence transcripts in HeLa mRNA. Proc Natl Acad Sci U S A. 1974; 71(5):1785-9. PMC: 388325. DOI: 10.1073/pnas.71.5.1785. View

Brawerman G, MENDECKI J, Lee S . A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry. 1972; 11(4):637-41. DOI: 10.1021/bi00754a027. View

Evans M, Lingrel J . Hemoglobin messenger ribonucleic acid. Synthesis of 9S and ribosomal ribonucleic acid during erythroid cell development. Biochemistry. 1969; 8(7):3000-5. DOI: 10.1021/bi00835a048. View

Searcy D, Macinnis A . Hybridization and renaturation of the nonrepetitive DNA of higher organisms. Biochim Biophys Acta. 1970; 209(2):574-7. DOI: 10.1016/0005-2787(70)90756-2. View

FREDERICQ E, Oth A, Fontaine F . The ultraviolet spectrum of deoxyribonucleic acids and their constituents. J Mol Biol. 1961; 3:11-7. DOI: 10.1016/s0022-2836(61)80003-x. View

Davidson E, HOUGH B, Amenson C, Britten R . General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus. J Mol Biol. 1973; 77(1):1-23. DOI: 10.1016/0022-2836(73)90359-8. View

10.

Wetmur J, Davidson N . Kinetics of renaturation of DNA. J Mol Biol. 1968; 31(3):349-70. DOI: 10.1016/0022-2836(68)90414-2. View

11.

Campo M, Bishop J . Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts. J Mol Biol. 1974; 90(4):649-63. DOI: 10.1016/0022-2836(74)90530-0. View

12.

Jacob E . Histone-gene reiteration in the genome of mouse. Eur J Biochem. 1976; 65(1):275-84. DOI: 10.1111/j.1432-1033.1976.tb10415.x. View

13.

Holmes D, Bonner J . Interspersion of repetitive and single-copy sequences in nuclear ribonucleic acid of high molecular weight. Proc Natl Acad Sci U S A. 1974; 71(4):1108-12. PMC: 388172. DOI: 10.1073/pnas.71.4.1108. View

14.

Britten R, Graham D, Neufeld B . Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974; 29:363-418. DOI: 10.1016/0076-6879(74)29033-5. View

15.

Greenberg J, PERRY R . Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA. J Cell Biol. 1971; 50(3):774-86. PMC: 2108299. DOI: 10.1083/jcb.50.3.774. View

16.

Rosbash M, Campo M, Gummerson K . Conservation of cytoplasmic poly (A)-containing RNA in mouse and rat. Nature. 1975; 258(5537):682-6. DOI: 10.1038/258682a0. View

17.

Wilson M, Melli M, Birnstiel M . Reiteration frequency of histone coding sequences in man. Biochem Biophys Res Commun. 1974; 61(2):404-9. DOI: 10.1016/0006-291x(74)90971-1. View

18.

Loening U . The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967; 102(1):251-7. PMC: 1270235. DOI: 10.1042/bj1020251. View

19.

Davidson E, HOUGH B, Chamberlin M, Britten R . Sequence repetition in the DNA of Nassaria (Iiyanassa) obsoleta. Dev Biol. 1971; 25(3):445-63. DOI: 10.1016/0012-1606(71)90041-8. View

20.

Marx K, Allen J, Hearst J . Characterization of the repetitious human DNA families. Biochim Biophys Acta. 1976; 425(2):129-47. DOI: 10.1016/0005-2787(76)90019-8. View