Lack of Agreement Among Two Commercial Enzyme-linked Immunosorbent Antibody Assays and a Conventional Immunofluorescence-based Method for Detecting Islet Cell Autoantibodies

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1996 Jul 1

PMID 8807208

Citations 1

Authors

E J Baron

D E Weber

L G Weide

Affiliations

Soon will be listed here.

Abstract

Two commercial enzyme-linked immunosorbent assays (ELISAs) for antibodies associated with development of insulin-dependent (type 1) diabetes mellitus (IDDM) were evaluated in conjunction with a conventional indirect immunofluorescent-antibody-islet cell antibody (ICA) test and a radioimmunoprecipitation method for detection of insulin autoantibodies in sera from a selected group of patients. The anti-ICA ELISA was positive for only 1 to 17 serum samples from newly diagnosed IDDM patients but yielded false-positive results with 2 of 6 serum samples containing non-diabetes-related autoantibodies. Although the anti-glutamic acid decarboxylase ELISA did not show positive results for sera with other autoantibodies, it was positive for only 4 of 29 serum samples from recently diagnosed IDDM patients and for 49% of 37 indirect immunofluorescent-antibody-ICA test-positive sera. Until the antibodies associated with the development of diabetes are better characterized, allowing better standards for comparison, it will be difficult to evaluate commercial assays in this field.

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Thorpe R, Swanson S Clin Diagn Lab Immunol. 2005; 12(1):28-39.

PMID: 15642981 PMC: 540193. DOI: 10.1128/CDLI.12.1.28-39.2005.

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