Chromosomal Heteromorphism Linked to the Mating Type Locus of the Oomycete Phytophthora Infestans

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1996 Aug 27

PMID 8804388

Citations 5

Authors

H S Judelson

Affiliations

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Abstract

The mating type locus of the oomycete, Phytophthora infestans, is embedded in a region of DNA that displays distorted and non-Mendelian segregation. By using DNA probes linked to the mating type locus to genetically and physically characterize that region, a large zone of chromosomal heteromorphism was detected. Locus S1 was shown to represent a tandemly repeated array of DNA that was typically present in a hemizygous state in A1 isolates while being absent from A2 isolates. The analysis of the parents and progeny of seven crosses indicated that the tandem array was linked in cis to the A1-determining allele of the mating type locus. A worldwide survey of genotypically diverse field isolates of P. infestans indicated that S1 was present in each of 48 isolates of the A1 mating type that were tested, but was absent in 46 of 47 A2 strains. Physical analysis of S1 indicated that the tandemly repeated DNA sequence spanned about 300 kb and had evolved from a 1.35-kb monomer. Internal deletions occurred within S1 during sexual propagation. This and other mutations apparently contributed to a high degree of polymorphism within the S1 array.

Citing Articles

Identification of the First Oomycete Mating-type Locus Sequence in the Grapevine Downy Mildew Pathogen, Plasmopara viticola.

Dussert Y, Legrand L, Mazet I, Couture C, Piron M, Serre R Curr Biol. 2020; 30(20):3897-3907.e4.

PMID: 32795448 PMC: 7116238. DOI: 10.1016/j.cub.2020.07.057.

Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating in .

Tzelepis G, Hoden K, Fogelqvist J, Asman A, Vetukuri R, Dixelius C Front Microbiol. 2020; 11:252.

PMID: 32153537 PMC: 7046690. DOI: 10.3389/fmicb.2020.00252.

Biologically active Phytophthora mating hormone prepared by catalytic asymmetric total synthesis.

Harutyunyan S, Zhao Z, den Hartog T, Bouwmeester K, Minnaard A, Feringa B Proc Natl Acad Sci U S A. 2008; 105(25):8507-12.

PMID: 18559862 PMC: 2438403. DOI: 10.1073/pnas.0709289105.

Amplification generates modular diversity at an avirulence locus in the pathogen Phytophthora.

Jiang R, Weide R, van de Vondervoort P, Govers F Genome Res. 2006; 16(7):827-40.

PMID: 16818726 PMC: 1484450. DOI: 10.1101/gr.5193806.

Genetic and physical variability at the mating type locus of the oomycete, Phytophthora infestans.

Judelson H Genetics. 1996; 144(3):1005-13.

PMID: 8913745 PMC: 1207597. DOI: 10.1093/genetics/144.3.1005.

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