Interaction of Calcium with Negative Lipids in Planar Bilayer Membranes. Influence of the Solvent

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1977 Aug 1

PMID 880335

Authors

J P Laclette

M Montal

Affiliations

Soon will be listed here.

Abstract

The interaction of Ca++ with acidic phospholipids in black lipid films and lipid bilayers formed from two monolayers was studied by measuring their physical stability and conductance. It was found that the addition of CaCl2 to only one side of lipid bilayers formed from phosphatidylserine or cardiolipin does not appreciably change these parameters. In contrast, black films are unstable to the asymmetric addition of CaCl2. Therefore, the destabilizing effect of Ca++ cannot be attributed to a surface charge difference. The only variation in composition between both bilayer membranes, namely the solvent content of the bilayer, seems to be responsible for the distinctive effect of Ca++. A tentative explanation is presented.

References

Papahadjopoulos D . Surface properties of acidic phospholipids: interaction of monolayers and hydrated liquid crystals with uni- and bi-valent metal ions. Biochim Biophys Acta. 1968; 163(2):240-54. DOI: 10.1016/0005-2736(68)90103-x. View

Montal M . Formation of bimolecular membranes from lipid monolayers. Methods Enzymol. 1974; 32:545-54. DOI: 10.1016/0076-6879(74)32053-8. View

Benz R, Frohlich O, Lauger P, Montal M . Electrical capacity of black lipid films and of lipid bilayers made from monolayers. Biochim Biophys Acta. 1975; 394(3):323-34. DOI: 10.1016/0005-2736(75)90287-4. View

Gitler C, Montal M . Formation of decane-soluble proteolipids: Influence of monovalent and divalent cations. FEBS Lett. 1972; 28(3):329-332. DOI: 10.1016/0014-5793(72)80743-9. View

Sherwood D, Montal M . Transmembrane lipid migration in planar asymmetric bilayer membranes. Biophys J. 2009; 15(5):417-34. PMC: 1334714. DOI: 10.1016/S0006-3495(75)85827-9. View

Montal M . Experimental membranes and mechanisms of bioenergy transductions. Annu Rev Biophys Bioeng. 1976; 5:119-75. DOI: 10.1146/annurev.bb.05.060176.001003. View

Montal M, Mueller P . Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A. 1972; 69(12):3561-6. PMC: 389821. DOI: 10.1073/pnas.69.12.3561. View

Tocanne J, VERVERGAERT P, Verkleij A, Van Deenen L . A monolayer and freeze-etching study of charged phospholipids. II. Ionic properties of mixtures of phosphatidylglycerol and lysylphosphatidylglycerol. Chem Phys Lipids. 1974; 12(3):220-31. DOI: 10.1016/0009-3084(74)90076-0. View

Mueller P, RUDIN D, Tien H, WESCOTT W . Reconstitution of cell membrane structure in vitro and its transformation into an excitable system. Nature. 1962; 194:979-80. DOI: 10.1038/194979a0. View

10.

Montal M . Asymmetric lipid bilayers. Reponse to multivalent ions. Biochim Biophys Acta. 1973; 298(3):750-4. DOI: 10.1016/0005-2736(73)90092-8. View

11.

Papahadjopoulos D, Ohki S . Stability of asymmetric phospholipid membranes. Science. 1969; 164(3883):1075-7. DOI: 10.1126/science.164.3883.1075. View