The Possible Role of Nitric Oxide in Relaxations and Excitatory Neuroeffector Transmission in the Cat Airway

Overview

Journal J Physiol

Specialty Physiology

Date 1996 Jun 15

PMID 8799899

Citations 2

Authors

H Tanaka

L Jing

S Takahashi

Y Ito

Affiliations

Soon will be listed here.

Abstract

1. To study the possible role of nitric oxide (NO free radical; NO) or NO-containing compounds in the non-adrenergic, non-cholinergic (NANC) relaxations, we observed the effects of carboxy-2-phenyl-4, 4, 5, 5-tetramethyl-imidazoline-1-oxyl-3-oxide (C-PTIO; a newly synthesized NO scavenger) on NANC relaxations in the cat airway. In addition, we also observed the effects of C-PTIO on excitatory junction potentials (EJPs), since NO has a prejunctional action on transmitter release. 2. Nitrosocystine (Cys-NO) (10(-7)-10(-3) M) dose-dependently relaxed the bronchial tissue in the presence of 5-HT, atropine and guanethidine and C-PTIO (10(-4) M) shifted the concentration-response curve of the Cys-NO to the right. 3. Electrical field stimulation (EFS) evoked biphasic NANC relaxations in the small bronchi of the cat. In general, C-PTIO suppressed non-selectively both the first and second components of the NANC relaxations to a similar extent. However, in some bronchial preparations C-PTIO (10(-4) M) selectively suppressed the first component of the NANC relaxation to approximately 50% of the initial value, enhancing the amplitude of the second component of the NANC relaxations. 4. After pretreatment of the bronchial tissues with alpha-chymotrypsin (1 unit ml-1) for 30 min in order to inhibit any response to peptides, EFS evoked monophasic NANC relaxation. C-PTIO (10(-5) - 10(-4) M) dose-dependently suppressed, and at a concentration of 10(-4) M almost halved, the amplitude of NANC relaxation. Additional application of L-NAME further reduced the C-PTIO-resistant NANC relaxation to 20-30% of the initial value. 5. C-PTIO (10(-4) M) enhanced the EJP amplitude evoked by single EFS in the trachea but not in the bronchi. However, C-PTIO enhanced the summation of the EJPs to repeated stimulation to a similar extent in the tracheal and bronchial tissues. Simultaneous application of C-PTIO and L-NAME did not further enhance the summation. 6. These results indicate that NO. and NO-containing compounds are involved in the L-NAME-sensitive NANC relaxation in the cat airway, and that only NO. has a prejunctional action which inhibits excitatory neuroeffector transmission.

Citing Articles

Spontaneous photo-relaxation of urethral smooth muscle from sheep, pig and rat and its relationship with nitrergic neurotransmission.

Triguero D, Costa G, Labadia A, Jimenez E, Garcia-Pascual A J Physiol. 2000; 522 Pt 3:443-56.

PMID: 10713968 PMC: 2269773. DOI: 10.1111/j.1469-7793.2000.00443.x.

Multiple calcium channels regulate neurotransmitter release from vagus nerve terminals in the cat bronchiole.

Fujisawa K, Onoue H, Abe K, Ito Y Br J Pharmacol. 1999; 128(1):262-8.

PMID: 10498861 PMC: 1571621. DOI: 10.1038/sj.bjp.0702791.

References

Inoue T, Ito Y . Characteristics of neuro-effector transmission in the smooth muscle layer of dog bronchiole and modifications by autacoids. J Physiol. 1986; 370:551-65. PMC: 1192696. DOI: 10.1113/jphysiol.1986.sp015950. View

Amis T, McKiernan B . Systematic identification of endobronchial anatomy during bronchoscopy in the dog. Am J Vet Res. 1986; 47(12):2649-57. View

Palmer R, Ferrige A, Moncada S . Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 1987; 327(6122):524-6. DOI: 10.1038/327524a0. View

Wolin M, Cherry P, Rodenburg J, Messina E, Kaley G . Methylene blue inhibits vasodilation of skeletal muscle arterioles to acetylcholine and nitric oxide via the extracellular generation of superoxide anion. J Pharmacol Exp Ther. 1990; 254(3):872-6. View

Hakoda H, Ito Y . Modulation of cholinergic neurotransmission by the peptide VIP, VIP antiserum and VIP antagonists in dog and cat trachea. J Physiol. 1990; 428:133-54. PMC: 1181639. DOI: 10.1113/jphysiol.1990.sp018204. View

Belvisi M, Stretton D, Barnes P . Nitric oxide as an endogenous modulator of cholinergic neurotransmission in guinea-pig airways. Eur J Pharmacol. 1991; 198(2-3):219-21. DOI: 10.1016/0014-2999(91)90626-2. View

Thornbury K, Ward S, Dalziel H, Carl A, Westfall D, Sanders K . Nitric oxide and nitrosocysteine mimic nonadrenergic, noncholinergic hyperpolarization in canine proximal colon. Am J Physiol. 1991; 261(3 Pt 1):G553-7. DOI: 10.1152/ajpgi.1991.261.3.G553. View

Xie Z, Hirose T, Hakoda H, Ito Y . Effects of vasoactive intestinal polypeptide antagonists on cholinergic neurotransmission in dog and cat trachea. Br J Pharmacol. 1991; 104(4):938-44. PMC: 1908857. DOI: 10.1111/j.1476-5381.1991.tb12530.x. View

Kosaka H, Watanabe M, Yoshihara H, Harada N, Shiga T . Detection of nitric oxide production in lipopolysaccharide-treated rats by ESR using carbon monoxide hemoglobin. Biochem Biophys Res Commun. 1992; 184(2):1119-24. DOI: 10.1016/0006-291x(92)90708-s. View

10.

Xie Z, Hakoda H, Ito Y . Airway epithelial cells regulate membrane potential, neurotransmission and muscle tone of the dog airway smooth muscle. J Physiol. 1992; 449:619-39. PMC: 1176098. DOI: 10.1113/jphysiol.1992.sp019105. View

11.

Buxton I, Cheek D, Eckman D, Westfall D, Sanders K, Keef K . NG-nitro L-arginine methyl ester and other alkyl esters of arginine are muscarinic receptor antagonists. Circ Res. 1993; 72(2):387-95. DOI: 10.1161/01.res.72.2.387. View

12.

Yoshida K, Akaike T, Doi T, Sato K, Ijiri S, Suga M . Pronounced enhancement of .NO-dependent antimicrobial action by an .NO-oxidizing agent, imidazolineoxyl N-oxide. Infect Immun. 1993; 61(8):3552-5. PMC: 281039. DOI: 10.1128/iai.61.8.3552-3555.1993. View

13.

Garcia-Pascual A, Triguero D . Relaxation mechanisms induced by stimulation of nerves and by nitric oxide in sheep urethral muscle. J Physiol. 1994; 476(2):333-47. PMC: 1160445. DOI: 10.1113/jphysiol.1994.sp020135. View

14.

Jing L, Inoue R, Tashiro K, Takahashi S, Ito Y . Role of nitric oxide in non-adrenergic, non-cholinergic relaxation and modulation of excitatory neuroeffector transmission in the cat airway. J Physiol. 1995; 483 ( Pt 1):225-37. PMC: 1157884. DOI: 10.1113/jphysiol.1995.sp020580. View

15.

Takahashi N, Tanaka H, Abdullah N, Jing L, Inoue R, Ito Y . Regional difference in the distribution of L-NAME-sensitive and -insensitive NANC relaxations in cat airway. J Physiol. 1995; 488 ( Pt 3):709-20. PMC: 1156736. DOI: 10.1113/jphysiol.1995.sp021002. View