Enumeration and Phylogenetic Analysis of Polycyclic Aromatic Hydrocarbon-degrading Marine Bacteria from Puget Sound Sediments

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1996 Sep 1

PMID 8795224

Citations 23

Authors

A D Geiselbrecht

R P Herwig

J W Deming

J T Staley

Affiliations

Soon will be listed here.

Abstract

Naphthalene- and phenanthrene-degrading bacteria in Puget Sound sediments were enumerated by most-probable-number enumeration procedures. Sediments from a creosote-contaminated Environmental Protection Agency Superfund Site (Eagle Harbor) contained from 10(4) to 10(7) polycyclic aromatic hydrocarbon (PAH)-degrading bacteria g (dry weight) of sediment-1, whereas the concentration at an uncontaminated site ranged from 10(3) to 10(4) g of sediment(-1). Isolates of PAH-degrading bacteria were obtained from these most-probable-number tubes as well as from sediment samples from noncontaminated sites and from bioreactors enriched with PAHs. The 18 resulting strains were grouped by whole-cell fatty acid analysis into two subgroups. The larger group of strains belonged to the newly described genus Cycloclasticus, whereas the other group contained members of the genus Vibrio. The Cycloclasticus group seems to be widespread in noncontaminated sediments. PAH degradation was confirmed in selected strains on the basis of removal of phenanthrene from growing cultures.

Citing Articles

Novel, active, and uncultured hydrocarbon-degrading microbes in the ocean.

Howe K, Zaugg J, Mason O Appl Environ Microbiol. 2024; 90(9):e0122424.

PMID: 39177328 PMC: 11409719. DOI: 10.1128/aem.01224-24.

Does with Its Bacterial Coating Represent a Promising Association for Seawater Phytoremediation of Diesel Hydrocarbons?.

Caronni S, Quaglini L, Franzetti A, Gentili R, Montagnani C, Citterio S Plants (Basel). 2023; 12(13).

PMID: 37447068 PMC: 10346704. DOI: 10.3390/plants12132507.

Complete Genome Sequence of sp. Strain PY97N, Which Includes Two Heavy Metal Resistance Genomic Islands.

Cui Z, Kivenson V, Liu N, Xu A, Luan X, Gao W Microbiol Resour Announc. 2019; 8(40).

PMID: 31582435 PMC: 6776764. DOI: 10.1128/MRA.00771-19.

Bacteriological quality, heavy metal and antibiotic resistance in Sapanca Lake, Turkey.

Ciftci Turetken P, Altug G, Cardak M, Gunes K Environ Monit Assess. 2019; 191(7):469.

PMID: 31243556 DOI: 10.1007/s10661-019-7588-8.

Biofilm-mediated enhanced crude oil degradation by newly isolated pseudomonas species.

Dasgupta D, Ghosh R, Sengupta T ISRN Biotechnol. 2015; 2013:250749.

PMID: 25937972 PMC: 4393046. DOI: 10.5402/2013/250749.

References

Garcia-Valdes E, Cozar E, Rotger R, Lalucat J, Ursing J . New naphthalene-degrading marine Pseudomonas strains. Appl Environ Microbiol. 1988; 54(10):2478-85. PMC: 204290. DOI: 10.1128/aem.54.10.2478-2485.1988. View

Deming J, Colwell R . Observations of barophilic microbial activity in samples of sediment and intercepted particulates from the demerara abyssal plain. Appl Environ Microbiol. 1985; 50(4):1002-6. PMC: 291783. DOI: 10.1128/aem.50.4.1002-1006.1985. View

Calder J, Lader J . Effect of dissolved aromatic hydrocarbons on the growth of marine bacteria in batch culture. Appl Environ Microbiol. 1976; 32(1):95-101. PMC: 170012. DOI: 10.1128/aem.32.1.95-101.1976. View

TAGGER S, Truffaut N, Le Petit J . Preliminary study on relationships among strains forming a bacterial community selected on naphthalene from a marine sediment. Can J Microbiol. 1990; 36(10):676-81. DOI: 10.1139/m90-115. View

Roubal G, Atlas R . Distribution of hydrocarbon-utilizing microorganisms and hydrocarbon biodegradation potentials in Alaskan continental shelf areas. Appl Environ Microbiol. 1978; 35(5):897-905. PMC: 242950. DOI: 10.1128/aem.35.5.897-905.1978. View

Atlas R . Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev. 1981; 45(1):180-209. PMC: 281502. DOI: 10.1128/mr.45.1.180-209.1981. View

Bazylinski D, Wirsen C, Jannasch H . Microbial utilization of naturally occurring hydrocarbons at the guaymas basin hydrothermal vent site. Appl Environ Microbiol. 1989; 55(11):2832-6. PMC: 203177. DOI: 10.1128/aem.55.11.2832-2836.1989. View

Tuveson R, Kagan J, Shaw M, Moresco G, von Behne E, Pu H . Phototoxic effects of fluoranthene, a polycyclic aromatic hydrocarbon, on bacterial species. Environ Mol Mutagen. 1987; 10(3):245-61. DOI: 10.1002/em.2850100304. View

Shiaris M, Cooney J . Replica plating method for estimating phenanthrene-utilizing and phenanthrene-cometabolizing microorganisms. Appl Environ Microbiol. 1983; 45(2):706-10. PMC: 242349. DOI: 10.1128/aem.45.2.706-710.1983. View

10.

West P, Okpokwasili G, Brayton P, Grimes D, Colwell R . Numerical taxonomy of phenanthrene-degrading bacteria isolated from the Chesapeake Bay. Appl Environ Microbiol. 1984; 48(5):988-93. PMC: 241663. DOI: 10.1128/aem.48.5.988-993.1984. View

11.

Larsen N, Olsen G, Maidak B, McCaughey M, Overbeek R, Macke T . The ribosomal database project. Nucleic Acids Res. 1993; 21(13):3021-3. PMC: 309730. DOI: 10.1093/nar/21.13.3021. View

12.

Guerin W, Jones G . Two-stage mineralization of phenanthrene by estuarine enrichment cultures. Appl Environ Microbiol. 1988; 54(4):929-36. PMC: 202575. DOI: 10.1128/aem.54.4.929-936.1988. View

13.

Gauthier M, LAFAY B, Christen R, Fernandez L, Acquaviva M, Bonin P . Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol. 1992; 42(4):568-76. DOI: 10.1099/00207713-42-4-568. View

14.

Foght J, Fedorak P, Westlake D . Mineralization of [14C]hexadecane and [14C]phenanthrene in crude oil: specificity among bacterial isolates. Can J Microbiol. 1990; 36(3):169-75. DOI: 10.1139/m90-030. View

15.

Sikkema J, de Bont J, Poolman B . Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev. 1995; 59(2):201-22. PMC: 239360. DOI: 10.1128/mr.59.2.201-222.1995. View

16.

Malins D, Krahn M, Brown D, Rhodes L, Myers M, McCain B . Toxic chemicals in marine sediment and biota from Mukilteo, Washington: relationships with hepatic neoplasms and other hepatic lesions in English sole (Parophrys vetulus). J Natl Cancer Inst. 1985; 74(2):487-94. View

17.

Dyksterhouse S, Gray J, Herwig R, Lara J, Staley J . Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int J Syst Bacteriol. 1995; 45(1):116-23. DOI: 10.1099/00207713-45-1-116. View

18.

Bogardt A, Hemmingsen B . Enumeration of phenanthrene-degrading bacteria by an overlayer technique and its use in evaluation of petroleum-contaminated sites. Appl Environ Microbiol. 1992; 58(8):2579-82. PMC: 195824. DOI: 10.1128/aem.58.8.2579-2582.1992. View

19.

Reichelt J, Baumann P, Baumann L . Study of genetic relationships among marine species of the genera Beneckea and Photobacterium by means of in vitro DNA/DNA hybridization. Arch Microbiol. 1976; 110(1):101-20. DOI: 10.1007/BF00416975. View

20.

Shiaris M . Seasonal Biotransformation of Naphthalene, Phenanthrene, and Benzo[a]pyrene in Surficial Estuarine Sediments. Appl Environ Microbiol. 1989; 55(6):1391-9. PMC: 202877. DOI: 10.1128/aem.55.6.1391-1399.1989. View