Study of Mother-to-child Epstein-Barr Virus Transmission by Means of Nested PCRs

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1996 Oct 1

PMID 8794321

Citations 11

Authors

M C Meyohas

V Marechal

N Desire

J Bouillie

J FROTTIER

J C Nicolas

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the possibility of vertical Epstein-Barr virus (EBV) transmission. We developed two nested-PCR methods for amplifying distinct regions of EBV DNA (BNRF1 and BamHI W) in circulating lymphocytes. Nested PCR was applied to samples obtained from 67 mother-infant pairs within 1 week of birth. We also tested samples from 16 neonates born to human immunodeficiency virus (HIV)-infected mothers to determine whether HIV increases the potential risk of vertical EBV transmission. About half of the 67 women in the first population were positive by nested PCR. Two neonates born to EBV PCR-positive women were also PCR positive. One of the 16 neonates born to HIV-infected women was PCR positive for EBV. These results strongly support the possibility of EBV transmission in utero or during delivery but do not suggest that HIV infection increases this risk. Further studies are required to confirm these findings, to identify the precise mode of vertical EBV transmission, and to determine the outcome for infants who are positive at birth for EBV DNA by nested PCR.

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References

Stagno S, Whitley R . Herpesvirus infections of pregnancy. Part II: Herpes simplex virus and varicella-zoster virus infections. N Engl J Med. 1985; 313(21):1327-30. DOI: 10.1056/NEJM198511213132105. View

Fleisher G, Bologonese R . Epstein-Barr virus infections in pregnancy: a prospective study. J Pediatr. 1984; 104(3):374-9. DOI: 10.1016/s0022-3476(84)81098-7. View

Gopal M, Thomson B, Fox J, Tedder R, Honess R . Detection by PCR of HHV-6 and EBV DNA in blood and oropharynx of healthy adults and HIV-seropositives. Lancet. 1990; 335(8705):1598-9. DOI: 10.1016/0140-6736(90)91433-b. View

Joab I, Triki H, de Saint Martin J, Perricaudet M, Nicolas J . Detection of anti-Epstein-Barr virus trans-activator (ZEBRA) antibodies in sera from patients with human immunodeficiency virus. J Infect Dis. 1991; 163(1):53-6. DOI: 10.1093/infdis/163.1.53. View

Rahman M, Kingsley L, ATCHISON R, Belle S, Breinig M, Ho M . Reactivation of Epstein-Barr virus during early infection with human immunodeficiency virus. J Clin Microbiol. 1991; 29(6):1215-20. PMC: 269972. DOI: 10.1128/jcm.29.6.1215-1220.1991. View

Marechal V, Nicolas J, Joab I, Costagliola D, Lefrere J . Epstein-Barr virus transactivator antibodies in HIV infection. Lancet. 1991; 338(8766):574. DOI: 10.1016/0140-6736(91)91140-p. View

Naher H, Gissmann L, Freese U, Petzoldt D, Helfrich S . Subclinical Epstein-Barr virus infection of both the male and female genital tract--indication for sexual transmission. J Invest Dermatol. 1992; 98(5):791-3. DOI: 10.1111/1523-1747.ep12499958. View

Schuster V, Janssen W, Seidenspinner S, Kreth H . [Congenital Epstein-Barr virus infection]. Monatsschr Kinderheilkd. 1993; 141(5):401-4. View

Telenti A, Uehlinger D, Marchesi F, Germann D, Malinverni R, Matter L . Epstein-Barr virus infection in HIV-positive patients. Eur J Clin Microbiol Infect Dis. 1993; 12(8):601-9. DOI: 10.1007/BF01973638. View

10.

Marechal V, Meyohas M, Joab I, Gaha S, Giot J, Sergeant A . Enzyme-linked immunosorbent assay for antibodies to ZEBRA, an Epstein-Barr trans-activator. Res Virol. 1993; 144(5):397-404. DOI: 10.1016/s0923-2516(06)80055-5. View

11.

Socie G, Gluckman E, Carosella E, Brossard Y, Lafon C, Brison O . Search for maternal cells in human umbilical cord blood by polymerase chain reaction amplification of two minisatellite sequences. Blood. 1994; 83(2):340-4. View

12.

Taylor Y, Melvin W, Sewell H, Flannelly G, Walker F . Prevalence of Epstein-Barr virus in the cervix. J Clin Pathol. 1994; 47(1):92-3. PMC: 501769. DOI: 10.1136/jcp.47.1.92. View

13.

Portnoy J, Ahronheim G, Ghibu F, CLECNER B, Joncas J . Recovery of Epstein-Barr virus from genital ulcers. N Engl J Med. 1984; 311(15):966-8. DOI: 10.1056/NEJM198410113111507. View

14.

Rickinson A, Yao Q, Wallace L . The Epstein-Barr virus as a model of virus-host interactions. Br Med Bull. 1985; 41(1):75-9. DOI: 10.1093/oxfordjournals.bmb.a072030. View

15.

Costa S, Barrasso R, Terzano P, Zerbini M, CARPI C, Musiani M . Detection of active Epstein-Barr infection in pregnant women. Eur J Clin Microbiol. 1985; 4(3):335-6. DOI: 10.1007/BF02013663. View

16.

Stagno S, Whitley R . Herpesvirus infections of pregnancy. Part I: Cytomegalovirus and Epstein-Barr virus infections. N Engl J Med. 1985; 313(20):1270-4. DOI: 10.1056/NEJM198511143132006. View

17.

Miyashita E, Yang B, Lam K, Crawford D, Thorley-Lawson D . A novel form of Epstein-Barr virus latency in normal B cells in vivo. Cell. 1995; 80(4):593-601. DOI: 10.1016/0092-8674(95)90513-8. View

18.

Yamamoto M, Kimura H, Hironaka T, Hirai K, Hasegawa S, Kuzushima K . Detection and quantification of virus DNA in plasma of patients with Epstein-Barr virus-associated diseases. J Clin Microbiol. 1995; 33(7):1765-8. PMC: 228265. DOI: 10.1128/jcm.33.7.1765-1768.1995. View

19.

Pearson H . PARTURITION VARICELLA-ZOSTER. Obstet Gynecol. 1964; 23:21-7. View

20.

Brunell P . Varicella-zoster infections in pregnancy. JAMA. 1967; 199(5):315-7. View