Molecular Cloning of the Lectin and a Lectin-related Protein from Common Solomon's Seal (Polygonatum Multiflorum)

Overview

Journal Plant Mol Biol

Date 1996 Jun 1

PMID 8790297

Citations 11

Authors

E J Van Damme

A Barre

P Rouge

F Van Leuven

J Balzarini

W J Peumans

Affiliations

Soon will be listed here.

Abstract

The most prominent protein of Polygonatum multiflorum (common Solomon's seal) rhizomes has been identified as a mannose-binding lectin. Analysis of the purified lectin demonstrated that it is a tetramer of four identical subunits of 14 kDa. Molecular cloning further revealed that the lectin from this typical Liliaceae species belongs to the superfamily of monocot mannose-binding proteins. Screening of cDNA libraries constructed with RNA isolated from buds, leaves and flowers of P. multiflorum also yielded cDNA clones encoding a protein, which contains two tandemly arranged domains with an obvious sequence homology to the mannose-binding lectins. Molecular modelling of the Polygonatum lectin and lectin-related protein indicated that the three-dimensional structure of both proteins strongly resembles that of the snowdrop lectin. In addition, this approach suggested that the presumed carbohydrate-binding sites of the lectin can accommodate a mannose residue whereas most of the carbohydrate-binding sites of the lectin-related protein cannot.

Citing Articles

Man-Specific Lectins from Plants, Fungi, Algae and Cyanobacteria, as Potential Blockers for SARS-CoV, MERS-CoV and SARS-CoV-2 (COVID-19) Coronaviruses: Biomedical Perspectives.

Barre A, Van Damme E, Simplicien M, Le Poder S, Klonjkowski B, Benoist H Cells. 2021; 10(7).

PMID: 34203435 PMC: 8305077. DOI: 10.3390/cells10071619.

Overview of the Structure⁻Function Relationships of Mannose-Specific Lectins from Plants, Algae and Fungi.

Barre A, Bourne Y, Van Damme E, Rouge P Int J Mol Sci. 2019; 20(2).

PMID: 30634645 PMC: 6359319. DOI: 10.3390/ijms20020254.

Distribution of Glycan Motifs at the Surface of Midgut Cells in the Cotton Leafworm () Demonstrated by Lectin Binding.

Walski T, De Schutter K, Cappelle K, Van Damme E, Smagghe G Front Physiol. 2017; 8:1020.

PMID: 29276491 PMC: 5727093. DOI: 10.3389/fphys.2017.01020.

Insights into animal and plant lectins with antimicrobial activities.

de Oliveira Dias R, Machado L, Migliolo L, Franco O Molecules. 2015; 20(1):519-41.

PMID: 25569512 PMC: 6272381. DOI: 10.3390/molecules20010519.

Anti-tumor and anti-viral activities of Galanthus nivalis agglutinin (GNA)-related lectins.

Wu L, Bao J Glycoconj J. 2012; 30(3):269-79.

PMID: 22893111 DOI: 10.1007/s10719-012-9440-z.

References

Van Damme E, Balzarini J, Smeets K, Van Leuven F, Peumans W . The monomeric and dimeric mannose-binding proteins from the Orchidaceae species Listera ovata and Epipactis helleborine: sequence homologies and differences in biological activities. Glycoconj J. 1994; 11(4):321-32. DOI: 10.1007/BF00731205. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Parker J, Guo D, Hodges R . New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. Biochemistry. 1986; 25(19):5425-32. DOI: 10.1021/bi00367a013. View

Peumans W, Allen A, Cammue B . A New Lectin from Meadow Saffron (Colchicum automnale). Plant Physiol. 1986; 82(4):1036-9. PMC: 1056254. DOI: 10.1104/pp.82.4.1036. View

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

Shibuya N, Goldstein I, Van Damme E, Peumans W . Binding properties of a mannose-specific lectin from the snowdrop (Galanthus nivalis) bulb. J Biol Chem. 1988; 263(2):728-34. View

von Heijne G . A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986; 14(11):4683-90. PMC: 311474. DOI: 10.1093/nar/14.11.4683. View

Van Damme E, Smeets K, Engelborghs I, Aelbers H, Balzarini J, Pusztai A . Cloning and characterization of the lectin cDNA clones from onion, shallot and leek. Plant Mol Biol. 1993; 23(2):365-76. DOI: 10.1007/BF00029011. View

Mirkov T, Wahlstrom J, Hagiwara K, Kjemtrup S, Chrispeels M . Evolutionary relationships among proteins in the phytohemagglutinin-arcelin-alpha-amylase inhibitor family of the common bean and its relatives. Plant Mol Biol. 1994; 26(4):1103-13. DOI: 10.1007/BF00040692. View

10.

Gaboriaud C, Bissery V, Benchetrit T, Mornon J . Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences. FEBS Lett. 1987; 224(1):149-55. DOI: 10.1016/0014-5793(87)80439-8. View

11.

Thornton J, Edwards M, Taylor W, Barlow D . Location of 'continuous' antigenic determinants in the protruding regions of proteins. EMBO J. 1986; 5(2):409-13. PMC: 1166746. DOI: 10.1002/j.1460-2075.1986.tb04226.x. View

12.

Oda Y, Minami K . Isolation and characterization of a lectin from tulip bulbs, Tulipa gesneriana. Eur J Biochem. 1986; 159(2):239-45. DOI: 10.1111/j.1432-1033.1986.tb09859.x. View

13.

Van Damme E, Goossens K, Smeets K, Van Leuven F, Verhaert P, Peumans W . The major tuber storage protein of araceae species is a lectin. Characterization and molecular cloning of the lectin from Arum maculatum L. Plant Physiol. 1995; 107(4):1147-58. PMC: 157247. DOI: 10.1104/pp.107.4.1147. View

14.

Saito K, Komae A, Kakuta M, Van Damme E, Peumans W, Goldstein I . The alpha-mannosyl-binding lectin from leaves of the orchid twayblade (Listera ovata). Application to separation of alpha-D-mannans from alpha-D-glucans. Eur J Biochem. 1993; 217(2):677-81. DOI: 10.1111/j.1432-1033.1993.tb18293.x. View

15.

Koike T, Titani K, Suzuki M, Beppu H, Kuzuya H, Maruta K . The complete amino acid sequence of a mannose-binding lectin from "Kidachi Aloe" (Aloe arborescens Miller var. natalensis Berger). Biochem Biophys Res Commun. 1995; 214(1):163-70. DOI: 10.1006/bbrc.1995.2270. View

16.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

17.

Smeets K, Van Damme E, Peumans W . Comparative study of the post-translational processing of the mannose-binding lectins in the bulbs of garlic (Allium sativum L.) and ramsons (Allium ursinum L.). Glycoconj J. 1994; 11(4):309-20. DOI: 10.1007/BF00731204. View

18.

Balzarini J, Naesens L, Slachmuylders J, Niphuis H, Rosenberg I, Holy A . 9-(2-Phosphonylmethoxyethyl)adenine (PMEA) effectively inhibits retrovirus replication in vitro and simian immunodeficiency virus infection in rhesus monkeys. AIDS. 1991; 5(1):21-8. DOI: 10.1097/00002030-199101000-00003. View

19.

Van Damme E, Barre A, Bemer V, Rouge P, Van Leuven F, Peumans W . A lectin and a lectin-related protein are the two most prominent proteins in the bark of yellow wood (Cladrastis lutea). Plant Mol Biol. 1995; 29(3):579-98. DOI: 10.1007/BF00020986. View

20.

Hester G, Kaku H, Goldstein I, Wright C . Structure of mannose-specific snowdrop (Galanthus nivalis) lectin is representative of a new plant lectin family. Nat Struct Biol. 1995; 2(6):472-9. DOI: 10.1038/nsb0695-472. View