Characterization of a Novel Anti-inflammatory Factor Produced by RM3/1 Macrophages Derived from Glucocorticoid Treated Human Monocytes

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 1995 Dec 1

PMID 8788234

Citations 9

Authors

W Hamann

A Floter

W Schmutzler

G Zwadlo-Klarwasser

Affiliations

Soon will be listed here.

Abstract

Glucocorticoids exert their anti-inflammatory activity by modulating the functions of various cell types including macrophages. They also induce the generation of a distinct macrophage subtype defined by the surface antigen RM3/1 which appears to be associated with the down-regulation of inflammation. Supernatants from these cells were found to exert a dose-dependent anti-inflammatory effect, particularly in the early phase as shown in the 5-hydroxytryptamine (5-HT) induced footpad edema of mice. By using conventional purification methods the anti-inflammatory factor was found to have a molecular mass of about 78 kD and an isoelectric point of about 7.9. Heat lability and sensitivity to trypsin and proteinase K indicate the protein nature of the anti-inflammatory factor. The inhibition of the early phase of inflammation and the molecular weight suggest that the anti-inflammatory agent released from RM3/1 macrophages is a novel protein different from other anti-inflammatory proteins described so far.

Citing Articles

CD163 as a Potential Biomarker of Monocyte Activation in Ischemic Stroke Patients.

Greco R, Demartini C, Zanaboni A, Tumelero E, Persico A, Candeloro E Int J Mol Sci. 2021; 22(13).

PMID: 34201498 PMC: 8268853. DOI: 10.3390/ijms22136712.

Decidua Basalis Mesenchymal Stem Cells Favor Inflammatory M1 Macrophage Differentiation In Vitro.

Abumaree M, Al Harthy S, Al Subayyil A, Alshabibi M, Abomaray F, Khatlani T Cells. 2019; 8(2).

PMID: 30781712 PMC: 6406276. DOI: 10.3390/cells8020173.

Human placental mesenchymal stem cells (pMSCs) play a role as immune suppressive cells by shifting macrophage differentiation from inflammatory M1 to anti-inflammatory M2 macrophages.

Abumaree M, Al Jumah M, Kalionis B, Jawdat D, Al Khaldi A, Abomaray F Stem Cell Rev Rep. 2013; 9(5):620-41.

PMID: 23812784 DOI: 10.1007/s12015-013-9455-2.

Differential expression of CD163 on monocyte subsets in healthy and HIV-1 infected individuals.

Tippett E, Cheng W, Westhorpe C, Cameron P, Brew B, Lewin S PLoS One. 2011; 6(5):e19968.

PMID: 21625498 PMC: 3098854. DOI: 10.1371/journal.pone.0019968.

Haptoglobin: A major susceptibility gene for diabetic vascular complications.

Szafranek T, Marsh S, Levy A Exp Clin Cardiol. 2009; 7(2-3):113-9.

PMID: 19649234 PMC: 2719180.

References

Wallner B, Mattaliano R, Hession C, Cate R, Tizard R, Sinclair L . Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature. 1986; 320(6057):77-81. DOI: 10.1038/320077a0. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Schleimer R . An overview of glucocorticoid anti-inflammatory actions. Eur J Clin Pharmacol. 1993; 45 Suppl 1:S3-7; discussion S43-4. DOI: 10.1007/BF01844196. View

Zwadlo G, Bruggen J, Gerhards G, Schlegel R, Sorg C . Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues. Clin Exp Immunol. 1988; 72(3):510-5. PMC: 1541584. View

Feige U, Overwien B, Sorg C . Purification of human blood monocytes by hypotonic density gradient centrifugation in Percoll. J Immunol Methods. 1982; 54(3):309-15. DOI: 10.1016/0022-1759(82)90315-5. View

Barnes P, Adcock I . Anti-inflammatory actions of steroids: molecular mechanisms. Trends Pharmacol Sci. 1993; 14(12):436-41. DOI: 10.1016/0165-6147(93)90184-l. View

Goulding N, Guyre P . Glucocorticoids, lipocortins and the immune response. Curr Opin Immunol. 1993; 5(1):108-13. DOI: 10.1016/0952-7915(93)90089-b. View

Zwadlo G, Voegeli R, Schulze Osthoff K, Sorg C . A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol. 1987; 55(6):295-304. DOI: 10.1159/000163432. View

Di Rosa M, Calignano A, Carnuccio R, Ialenti A, Sautebin L . Multiple control of inflammation by glucocorticoids. Agents Actions. 1986; 17(3-4):284-9. DOI: 10.1007/BF01982621. View

10.

Flower R . Eleventh Gaddum memorial lecture. Lipocortin and the mechanism of action of the glucocorticoids. Br J Pharmacol. 1988; 94(4):987-1015. PMC: 1854095. DOI: 10.1111/j.1476-5381.1988.tb11614.x. View

11.

Oyanagui Y, Suzuki S . Vasoregulin, a glucocorticoid-inducible vascular permeability inhibitory protein. Agents Actions. 1986; 17(3-4):270-7. DOI: 10.1007/BF01982619. View

12.

Flower R, Rothwell N . Lipocortin-1: cellular mechanisms and clinical relevance. Trends Pharmacol Sci. 1994; 15(3):71-6. DOI: 10.1016/0165-6147(94)90281-x. View

13.

Zwadlo-Klarwasser G, Bent S, Haubeck H, Sorg C, Schmutzler W . Glucocorticoid-induced appearance of the macrophage subtype RM 3/1 in peripheral blood of man. Int Arch Allergy Appl Immunol. 1990; 91(2):175-80. DOI: 10.1159/000235111. View

14.

Guyre P, Girard M, Morganelli P, Manganiello P . Glucocorticoid effects on the production and actions of immune cytokines. J Steroid Biochem. 1988; 30(1-6):89-93. DOI: 10.1016/0022-4731(88)90080-5. View

15.

Carnuccio R, Di Rosa M, Guerrasio B, Iuvone T, Sautebin L . Vasocortin: a novel glucocorticoid-induced anti-inflammatory protein. Br J Pharmacol. 1987; 90(3):443-5. PMC: 1917188. DOI: 10.1111/j.1476-5381.1987.tb11193.x. View

16.

Raynal P, Pollard H . Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteins. Biochim Biophys Acta. 1994; 1197(1):63-93. DOI: 10.1016/0304-4157(94)90019-1. View

17.

Sautebin L, Carnuccio R, Ialenti A, Di Rosa M . Lipocortin and vasocortin: two species of anti-inflammatory proteins mimicking the effects of glucocorticoids. Pharmacol Res. 1992; 25(1):1-12. DOI: 10.1016/s1043-6618(05)80058-1. View