Lamellar Stacking in Three-dimensional Crystals of Ca(2+)-ATPase from Sarcoplasmic Reticulum

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1996 Apr 1

PMID 8785327

Citations 3

Authors

G W Cheong

H S Young

H Ogawa

C Toyoshima

D L Stokes

Affiliations

Soon will be listed here.

Abstract

Electron microscopy of multilamellar crystals of CA(2+)-ATPase currently offers the best opportunity for obtaining a high-resolution structure of this ATP-driven ion pump. Under certain conditions small, wormlike crystals are formed and provide views parallel to the lamellar plane, from which parameters of lamellar stacking can be directly measured. Assuming that molecular packing is the same, data from these views could supplement those obtained by tilting large, thin platelike crystals. However, we were surprised to discover that the lamellar spacing was variable and depended on the amount of glycerol present during crystallization (20% versus 5%). Projection maps (h,0,l) from these womklike crystals suggest different molecular contacts that give rise to the different lamellar spacings. Based on an orthogonal projection map (h,k,0) from collapsed, wormlike crystals and on x-ray powder patterns, we conclude that molecular packing within the lamellar plane is the same as that in thin, platelike crystals and is unaffected by glycerol. Finally, the orientation of molecules in the lamellar plane was characterized from freeze-dried, shadowed crystals. Comparing the profile of molecules in these multilamellar crystals with that previously observed in helical tubes induced by vanadate gives structural evidence of the conformational change that accompanies binding of calcium of Ca(2+)-ATPase.

Citing Articles

Modeling a dehalogenase fold into the 8-A density map for Ca(2+)-ATPase defines a new domain structure.

Stokes D, Green N Biophys J. 2000; 78(4):1765-76.

PMID: 10733958 PMC: 1300772. DOI: 10.1016/s0006-3495(00)76727-0.

Two-dimensional crystallization of Ca-ATPase by detergent removal.

Lacapere J, Stokes D, Olofsson A, RIGAUD J Biophys J. 1998; 75(3):1319-29.

PMID: 9726933 PMC: 1299806. DOI: 10.1016/S0006-3495(98)74050-0.

Structure of the Ca2+ pump of sarcoplasmic reticulum: a view along the lipid bilayer at 9-A resolution.

Ogawa H, Stokes D, Sasabe H, Toyoshima C Biophys J. 1998; 75(1):41-52.

PMID: 9649366 PMC: 1299678. DOI: 10.1016/S0006-3495(98)77493-4.

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