Phylogenetic Compositions of Bacterioplankton from Two California Estuaries Compared by Denaturing Gradient Gel Electrophoresis of 16S RDNA Fragments

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1996 Jul 1

PMID 8779608

Citations 65

Authors

A E Murray

J T Hollibaugh

C Orrego

Affiliations

Soon will be listed here.

Abstract

The phylogenetic compositions of bacterioplankton assemblages from San Francisco Bay and Tomales Bay, Calif., differed substantially when analyzed by PCR-denaturing gradient gel electrophoresis; these differences are consistent with the results of previous studies demonstrating differences in their metabolic capabilities. PCR-denaturing gradient gel electrophoresis analysis of complex microbial assemblages was sensitive and reliable, and the results were reproducible as shown by experiments with constructed and naturally occurring assemblages.

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