In Vitro Effect of Ciprofloxacin on HT-29 Human Colon Carcinoma Cell Line: Assessment of Cell Proliferation by Thymidine Uptake and Silver Nucleolar Organizer Regions (AgNOR) Histomorphometry

Overview

Journal Arch Toxicol

Specialty Toxicology

Date 1995 Jan 1

PMID 8773185

Citations 2

Authors

M Rabau

A Nyska

D Dayan

Affiliations

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Abstract

In an in vitro study, the effect of ciprofloxacin was examined on proliferation of HT-29 human colon carcinoma cell line. Cell proliferation was measured by 3H-thymidine uptake and silver nucleolar organizer region (AgNOR) histomorphometry. A significant increase in [3H]thymidine incorporation (p < 0.05) parallel with a significant increase in the mean AgNOR clusters/cells (p < 0.01) was noted after 5 days of cell exposure to ciprofloxacin. It is suggested that the assessment of AgNOR variables is useful in examination of quinolone effect on HT-29 human colon carcinoma cell proliferation.

Citing Articles

Effect of Ciprofloxacin on Susceptibility to Aortic Dissection and Rupture in Mice.

LeMaire S, Zhang L, Luo W, Ren P, Azares A, Wang Y JAMA Surg. 2018; 153(9):e181804.

PMID: 30046809 PMC: 6233654. DOI: 10.1001/jamasurg.2018.1804.

Ciprofloxacin decreases survival in HT-29 cells via the induction of TGF-beta1 secretion and enhances the anti-proliferative effect of 5-fluorouracil.

Bourikas L, Kolios G, Valatas V, Notas G, Drygiannakis I, Pelagiadis I Br J Pharmacol. 2009; 157(3):362-70.

PMID: 19371339 PMC: 2707983. DOI: 10.1111/j.1476-5381.2009.00161.x.

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