Inhibition of Protein Synthesis in Frog (Xenopus Laevis) Egg Extracts by an Antibody Against Phosphatidylinositol 4,5-bisphosphate

Overview

Journal Biochem J

Specialty Biochemistry

Date 1996 Aug 1

PMID 8760344

Citations 1

Authors

T J Keating

K Fukami

K R Robinson

Affiliations

Soon will be listed here.

Abstract

The antibody kt10, which is directed against the phospholipid PtdIns(4,5)P2, inhibits protein synthesis when added to cytosolic extracts prepared from frog eggs. Addition of stable analogues of diacylglycerol and Ins(1,4,5)P3 failed to rescue the inhibition of translation, suggesting that the effect of the antibody was not to block hydrolysis of PtdIns(4,5)P2. Neomycin, which also binds PtdIns(4,5)P2, produced a similar reduction in protein-synthesis levels in the extract system, supporting the idea that it is the interaction of the antibody with PtdIns(4,5)P2 that is producing the effect.

Citing Articles

Phosphatidylinositol transfer proteins and protein kinase C make separate but non-interacting contributions to the phosphorylation state necessary for secretory competence in rat mast cells.

Pinxteren J, Gomperts B, Rogers D, Phillips S, Tatham P, Thomas G Biochem J. 2001; 356(Pt 1):287-96.

PMID: 11336662 PMC: 1221838. DOI: 10.1042/0264-6021:3560287.

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