Dietary Eritadenine-induced Alteration of Molecular Species Composition of Phospholipids in Rats

Overview

Journal Lipids

Specialty Biochemistry

Date 1996 Apr 1

PMID 8743052

Citations 4

Authors

K Sugiyama

A Yamakawa

Affiliations

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Abstract

The effect of dietary eritadenine, a hypocholesterolemic compound found in the mushroom Lentinus edodes, on the fatty acid and molecular species profiles of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in the plasma and liver microsomes was investigated in relation to the hypocholesterolemic action of the compound in rats. Animals were fed the control or eritadenine-supplemented (50 mg/kg diet) diet for 14 d. Eritadenine supplementation significantly decreased the plasma concentrations of cholesterol and phospholipids, but not triglycerides. The PC/PE ratio of liver microsomes, but not plasma, was also markedly decreased by eritadenine. Eritadenine supplementation was found to increase the proportion of 18:2n-6 and, inversely, to decrease the proportion of 20:4n-6 and 22:5n-6 in plasma PC and liver microsomal PC and PE, indicating that eritadenine depressed the metabolism of linoleic acid. The effect of eritadenine on the profile of n-3 fatty acids was dissimilar in PC and PE. These changes in fatty acid composition were selectively reflected in the molecular species composition of both PC and PE; the extent of increase in 16:0-18:2 molecular species or decrease in 18:0-20:4 molecular species was apparently greater than that of other molecular species containing 18:2 or 20:4 in the sn-2 position. These results suggest that, in addition to the decrease in liver microsomal PC/PE ratio, the alteration of plasma PC molecular species composition might also participate in the hypocholesterolemic action of eritadenine.

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References

Leikin A, Brenner R . In vivo phospholipid modification induces changes in microsomal delta 5-desaturase activity. Biochim Biophys Acta. 1992; 1165(2):189-93. DOI: 10.1016/0005-2760(92)90186-y. View

BARTLETT G . Phosphorus assay in column chromatography. J Biol Chem. 1959; 234(3):466-8. View

Chibata I, Okumura K, Takeyama S, Kotera K . Lentinacin: a new hypocholesterolemic substance in Lentinus edodes. Experientia. 1969; 25(12):1237-8. DOI: 10.1007/BF01897467. View

Takashima K, Sato C, Sasaki Y, Morita T, Takeyama S . Effect of eritadenine on cholesterol metabolism in the rat. Biochem Pharmacol. 1974; 23(2):433-8. DOI: 10.1016/0006-2952(74)90434-1. View

Fletcher M . A colorimetric method for estimating serum triglycerides. Clin Chim Acta. 1968; 22(3):393-7. DOI: 10.1016/0009-8981(68)90041-7. View

Kinsella J, Broughton K, WHELAN J . Dietary unsaturated fatty acids: interactions and possible needs in relation to eicosanoid synthesis. J Nutr Biochem. 1990; 1(3):123-41. DOI: 10.1016/0955-2863(90)90011-9. View

Sundler R, Akesson B . Regulation of phospholipid biosynthesis in isolated rat hepatocytes. Effect of different substrates. J Biol Chem. 1975; 250(9):3359-67. View

Leduc R, Patton G, Atkinson D, Robins S . Influence of different molecular species of phosphatidylcholine on cholesterol transport from lipoprotein recombinants in the rat. J Biol Chem. 1987; 262(16):7680-5. View

Vance J . The use of newly synthesized phospholipids for assembly into secreted hepatic lipoproteins. Biochim Biophys Acta. 1989; 1006(1):59-69. DOI: 10.1016/0005-2760(89)90323-8. View

10.

Leikin A, Brenner R . Cholesterol-induced microsomal changes modulate desaturase activities. Biochim Biophys Acta. 1987; 922(3):294-303. DOI: 10.1016/0005-2760(87)90052-x. View

11.

Blank M, Robinson M, Fitzgerald V, Snyder F . Novel quantitative method for determination of molecular species of phospholipids and diglycerides. J Chromatogr. 1984; 298(3):473-82. DOI: 10.1016/s0021-9673(01)92744-x. View

12.

Leikin A, Brenner R . In vivo cholesterol removal from liver microsomes induces changes in fatty acid desaturase activities. Biochim Biophys Acta. 1988; 963(2):311-9. DOI: 10.1016/0005-2760(88)90296-2. View

13.

Kadowaki H, Patton G, Robins S . Effect of phosphatidylcholine molecular species on the uptake of HDL triglycerides and cholesteryl esters by the liver. J Lipid Res. 1993; 34(2):180-9. View

14.

Takashima K, Izumi K, Iwai H, Takeyama S . The hypocholesterolemic action of eritadenine in the rat. Atherosclerosis. 1973; 17(3):491-502. DOI: 10.1016/0021-9150(73)90039-7. View

15.

Wu A, WINDMUELLER H . Relative contributions by liver and intestine to individual plasma apolipoproteins in the rat. J Biol Chem. 1979; 254(15):7316-22. View

16.

ROKUJO T, Kikuchi H, Tensho A, Tsukitani Y, Takenawa T, Yoshida K . Lentysine: a new hypolipidemic agent from a mushroom. Life Sci. 1970; 9(7):379-85. DOI: 10.1016/0024-3205(70)90240-7. View

17.

Kadowaki H, Patton G, Robins S . Metabolism of high density lipoprotein lipids by the rat liver: evidence for participation of hepatic lipase in the uptake of cholesteryl ester. J Lipid Res. 1992; 33(11):1689-98. View

18.

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

19.

Schanche J, Schanche T, Ueland P, Holy A, Votruba I . The effect of aliphatic adenine analogues on S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase in intact rat hepatocytes. Mol Pharmacol. 1984; 26(3):553-8. View

20.

Imaizumi K, Mawatari K, Murata M, Ikeda I, Sugano M . The contrasting effect of dietary phosphatidylethanolamine and phosphatidylcholine on serum lipoproteins and liver lipids in rats. J Nutr. 1983; 113(12):2403-11. DOI: 10.1093/jn/113.12.2403. View