Orientation of Myelin Proteolipid Protein in the Oligodendrocyte Cell Membrane

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1996 Apr 1

PMID 8734436

Citations 5

Authors

J M Greer

C A DYER

M Pakaski

C Symonowicz

M B LEES

Affiliations

Soon will be listed here.

Abstract

The orientation of proteins within a cell membrane can often be difficult to determine. A number of models have been proposed for the orientation of the myelin protein, proteolipid protein (PLP), each of which includes exposed domains on the intracellular and extracellular membrane faces. Immunolabeling experiments have localized the C-terminus and the region spanning amino acids 103-116 to the cytoplasmic face of the membrane, but no well characterized antibodies have been available that label extracellular PLP domains. In this report, we describe the generation and characterization of mouse monoclonal antibodies (mAb) against putative extramembrane domains. Three of the mAb, specific for PLP peptides 40-59, 178-191, or 215-232, immunostain live oligodendrocytes, indicating that these regions of the molecule are exposed on the external surface of the cell. In addition, we have used these mAb to study the time-course of incorporation of PLP into the oligodendrocyte membrane. These studies increase our knowledge of the orientation of PLP in the lipid bilayer and are relevant for understanding myelin function.

Citing Articles

Correlation Between Anti-Myelin Proteolipid Protein (PLP) Antibodies and Disease Severity in Multiple Sclerosis Patients With PLP Response-Permissive HLA Types.

Greer J, Trifilieff E, Pender M Front Immunol. 2020; 11:1891.

PMID: 32973782 PMC: 7473150. DOI: 10.3389/fimmu.2020.01891.

A common mechanism of PLP/DM20 misfolding causes cysteine-mediated endoplasmic reticulum retention in oligodendrocytes and Pelizaeus-Merzbacher disease.

Dhaunchak A, Nave K Proc Natl Acad Sci U S A. 2007; 104(45):17813-8.

PMID: 17962415 PMC: 2077047. DOI: 10.1073/pnas.0704975104.

Schwann cell expression of PLP1 but not DM20 is necessary to prevent neuropathy.

Shy M, Hobson G, Jain M, Boespflug-Tanguy O, Garbern J, Sperle K Ann Neurol. 2003; 53(3):354-65.

PMID: 12601703 PMC: 4744322. DOI: 10.1002/ana.10466.

A history of proteolipids: a personal memoir.

LEES M Neurochem Res. 1998; 23(3):261-71.

PMID: 9482238 DOI: 10.1023/a:1022488912996.

Conservation of topology, but not conformation, of the proteolipid proteins of the myelin sheath.

Gow A, Gragerov A, Gard A, Colman D, Lazzarini R J Neurosci. 1997; 17(1):181-9.

PMID: 8987747 PMC: 6793710.

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