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Identification of Glycoproteins on Nitrocellulose Membranes and Gels

Overview
Journal Mol Biotechnol
Publisher Springer
Specialties Biotechnology
Molecular Biology
Date 1996 Apr 1
PMID 8734429
Citations 20
Authors
D J Thornton
I Carlstedt
J K Sheehan
Affiliations
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Abstract

In this article we describe a procedure for the detection of glycoproteins on gels employing the periodic acid-Schiff's reagent. In addition, a number of staining protocols and direct binding ELISA, employing antibodies and lectins, are described for the identification and quantitation of glycoproteins after their immobilization by dot, slot, or Western blotting onto nitrocellulose membranes. We document, in detail, the conditions (i.e., the effect of solvent and detergents) for the immobilization of one specific family of O-linked glycoproteins, namely mucins. However, taking into account our suggestions, these procedures should be applicable to other types of glycoprotein.

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