The Organization of DNA Sequences in the Mouse Genome

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1977 May 23

PMID 872701

Citations 6

Authors

E Ginelli

R Di Lernia

G Corneo

Affiliations

Soon will be listed here.

Abstract

Analysis of the organization of nucleotide sequences in mouse genome is carried out on total DNA at different fragment size, reannealed to intermediate value of Cot, by Ag+--Cs2SO4 density gradient centrifugation.--According to nuclease S-1 resistance and kinetic renaturation curves mouse genome appears to be made up of non-repetitive DNA (76% of total DNA), middle repetitive DNA (average repetition frequency 2X10(4) copies, 15% of total DNA), highly repetitive DNA (8% of total DNA) and fold-back DNA (renatured density 1.701 g/ml, 1% of total DNA).--Non-repetitive sequences are intercalated with short middle repetitive sequences. One third of non-repetitive sequences is longer than 4500 nucleotides, another third is long between 1800 and 4500 nucleotides, and the remainder is shorter than 1800 nucleotides.--Middle repetitive sequences are transcribed in vivo. The majority of the transcribed repeated sequences appears to be not linked to the bulk of non-repeated sequences at a DNA size of 1800 nucleotides.--The organization of mouse genome analyzed by Ag+--Cs2SO4 density gradient of reannealed DNA appears to be substantially different than that previously observed in human genome using the same technique.

Citing Articles

Locations of three repetitive sequence families found in BALB/c adult beta-globin clones.

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Sequence organization in the DNA of three Selachians.

Olmo E, Stingo V, Odierna G, Capriglione T Experientia. 1982; 38(3):339-40.

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Sequence organization of animal nuclear DNA.

Schmidtke J, Epplen J Hum Genet. 1980; 55(1):1-18.

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Satellite DNA sequences and reproductive isolation in the Drosophila willistoni group.

Corneo G, Nelli L, Meazza D, Ayala F Experientia. 1980; 36(7):837-9.

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Absence of satellite DNA synthesis during meiotic prophase in mouse and human spermatocytes.

Hotta Y, Stern H Chromosoma. 1978; 69(3):323-30.

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