Antagonistic Activity of Poly (4-vinylpyridine-N-oxide) to the Inhibition of Viral Interferon Induction by Asbestos Fibres

Overview

Journal Br J Ind Med

Specialty Occupational Medicine

Date 1977 May 1

PMID 871442

Authors

N Hahon

J A Booth

H L ECKERT

Affiliations

Soon will be listed here.

Abstract

The depressive activity of both serpentine (Canadian and Rhodesian chrysotiles) and amphibole (amosite, crocidolite, and anthophyllite) asbestos fibres on interferon induction by influenza virus was significantly diminished or abolished completely when either asbestos fibres or LLC-MK2 cell monolayers were pretreated with poly(4-vinylpyridine-N-oxide). Maximal antagonistic activity of the polymer was time and concentration dependent. Pretreating asbestos fibres with the polymer was more rapid and effective in encouraging viral interferon synthesis than pretreating cell monolayers. Virus multiplication in the presence of asbestos fibre-treated cell monolayers attained a twofold higher level than that noted in normal cell monolayers or those containing polymer-pretreated asbestos fibres. These findings were related to the suppression of interferon production.

References

Beck E, Bruch J, Brockhaus A . [Alteration of the cytotoxic effect of quartz on mouse fibroblasts (strain L) by polyvinylpyridine-N-oxide]. Z Zellforsch Mikrosk Anat. 1963; 59:568-76. View

SCHLIPKOETER H, Brockhaus A . [Inhibition of experimental silicosis by subcutaneous administration of polyvinylpyridine-N-oxide]. Klin Wochenschr. 1961; 39:1182-9. DOI: 10.1007/BF01532453. View

Harington J, Allison A, Badami D . Mineral fibers: chemical, physicochemical, and biological properties. Adv Pharmacol Chemother. 1975; 12(0):291-402. DOI: 10.1016/s1054-3589(08)60223-9. View

GAINER J . Increased mortality in encephalomyocarditis virus-infected mice consuming cobalt sulfate: tissue concentrations of cobalt. Am J Vet Res. 1972; 33(10):2067-73. View

Hahan N . Depression of viral interferon induction in cell monolayers by coal dust. Br J Ind Med. 1974; 31(3):201-8. PMC: 1009585. View

Allison A . Effects of silica and asbestos on cells in culture. Inhaled Part. 1970; 1:437-45. View

SCHNITZER R . Modification of biological surface activity of particles. Environ Health Perspect. 1974; 9:261-6. PMC: 1475386. DOI: 10.1289/ehp.749261. View

SCHNITZER R, BUNESCU G, Baden V . Interactions of mineral fiber surfaces with cells in vitro. Ann N Y Acad Sci. 1971; 172(23):759-72. View

Davis J . The effects of polyvinyl pyridine-N-oxide (P204) on the cytopathogenic action of chrysotile asbestos in vivo and in vitro. Br J Exp Pathol. 1972; 53(6):652-8. PMC: 2072487. View

10.

GAINER J . Effects of arsenicals on interferon formation and action. Am J Vet Res. 1972; 33(12):2579-86. View

11.

BAIRATI A, Castano P . [An electron microscopy study of the localization of synthetic, cytoprotective polymers against silica in the mouse liver (polyvinyl pridine-N-oxide and poly-p-dimethylaminostryrene-N-oxide)]. Med Lav. 1968; 59(2):81-104. View

12.

MACNAB G, Harington J . Haemolytic activity of asbestos and other mineral dusts. Nature. 1967; 214(5087):522-3. DOI: 10.1038/214522a0. View

13.

Comolli R . Cytotoxicity of silica and liberation of lysosomal enzymes. J Pathol Bacteriol. 1967; 93(1):241-53. DOI: 10.1002/path.1700930124. View

14.

Nash T, Allison A, Harington J . Physico-chemical properties of silica in relation to its toxicity. Nature. 1966; 210(5033):259-61. DOI: 10.1038/210259a0. View

15.

Hahon N, Booth J, ECKERT H . Interferon assessment by the immunofluorescent, immunoperoxidase, and hemadsorption cell-counting techniques. Arch Virol. 1975; 48(3):239-43. DOI: 10.1007/BF01317966. View

16.

Hahon N . Counteraction of poly(4-vinylpyridine-n-oxide) on the depression of viral interferon induction by coal dust. Infect Immun. 1976; 13(5):1334-42. PMC: 420761. DOI: 10.1128/iai.13.5.1334-1342.1976. View

17.

Hahon N, Booth J, ECKERT H . Cell attachment and penetration by influenza virus. Infect Immun. 1973; 7(3):341-51. PMC: 422681. DOI: 10.1128/iai.7.3.341-351.1973. View

18.

Richards R, Hext P, Blundell G, Henderson W, Volcani B . Ultrastructural changes in lung fibroblast cultures exposed to chrysotile asbestos. Br J Exp Pathol. 1974; 55(3):275-81. PMC: 2072625. View

19.

Kaw J, Beck E, Bruch J . Studies of quartz cytotoxicity on peritoneal macrophages of guinea pigs pretreated with polyvinylpyridine N-oxide. Environ Res. 1975; 9(3):313-20. DOI: 10.1016/0013-9351(75)90011-0. View

20.

Harington J, Miller K, MACNAB G . Hemolysis by asbestos. Environ Res. 1971; 4(2):95-117. DOI: 10.1016/0013-9351(71)90038-7. View